Description

H-Leu-Val-Tyr-Amc CAS NO 76524-85-1 is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly for enzymes like chymotrypsin and subtilisin. It is an essential tool for biochemical research, pharmaceutical development, and diagnostic applications where accurate kinetic analysis is paramount.

Application

• Protease Activity Assays: Primary substrate for the fluorometric determination of chymotrypsin, subtilisin, and related serine proteases.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and mechanistic investigations of protease function.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for detecting and quantifying specific protease levels in clinical samples.
• Quality Control (QC): Utilized in biopharmaceutical QC labs to monitor the activity and consistency of enzyme-based products and processes.

Basic Information

Product Name	H-Leu-Val-Tyr-Amc
CAS No.	76524-85-1
Molecular Formula	C27H35N5O5
Molecular Weight	509.60 g/mol
Synonyms	L-Leucyl-L-valyl-L-tyrosine 7-amido-4-methylcoumarin; Leu-Val-Tyr-AMC; LVY-AMC; L-Leu-L-Val-L-Tyr-AMC; Chymotrypsin Substrate I (AMC); Suc-Leu-Val-Tyr-AMC (related); Protease Fluorogenic Substrate; 7-Amido-4-methylcoumarin conjugate of Leu-Val-Tyr
EINECS	Contact for details

Quality Control

Our H-Leu-Val-Tyr-Amc is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, essential for reliable assay performance. Each lot is characterized by advanced analytical techniques including HPLC, MS, and NMR to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Tested for chymotrypsin hydrolysis

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.