Description

4',5'-Dimethoxy-6-Carboxyfluorescein is a chemically modified, carboxylated derivative of fluorescein, designed for advanced conjugation and labeling applications. This compound is valued for its enhanced photostability and specific reactivity, making it a superior choice for creating stable, fluorescent probes. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, diagnostic assay development, and advanced fluorescence microscopy.

Application

• Bioconjugation and Probe Synthesis: Acts as a key intermediate for labeling antibodies, proteins, peptides, and oligonucleotides with a fluorescent tag.
• Diagnostic Assay Development: Used in the creation of fluorescent substrates and tracers for ELISA, lateral flow assays, and other immunoassays.
• Cell Biology and Microscopy: Employed in the synthesis of fluorescent dyes for cell staining, intracellular pH sensing, and live-cell imaging applications.
• Flow Cytometry: Serves as a precursor for developing bright and photostable fluorophores for cell sorting and analysis.
• Chemical Biology Research: Utilized as a building block for creating activity-based probes and sensors to study enzymatic activity and biomolecular interactions.
• Material Science: Can be incorporated into polymers or nanoparticles to create functional, fluorescent materials for sensing and imaging.

Basic Information

Product Name	4',5'-Dimethoxy-6-Carboxyfluorescein
CAS No.	75929-58-7
Molecular Formula	C25H18O9
Molecular Weight	462.41 g/mol
Synonyms	6-Carboxy-4',5'-dimethoxyfluorescein; 6-CF (6-Carboxyfluorescein) dimethoxy derivative; DMF-6-COOH; 2-(6-Carboxy-3-oxo-3H-xanthen-9-yl)-4,5-dimethoxybenzoic acid; 4',5'-Dimethoxyfluorescein-6-carboxylic acid; 6-Carboxy-2',7'-dimethoxyfluorescein (common numbering variation); Fluorescein, 6-carboxy-4',5'-dimethoxy-
EINECS	Contact for details

Quality Control

Our 4',5'-Dimethoxy-6-Carboxyfluorescein is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry to verify optical properties. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and manufacturing specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Max (λmax)	~495-505 nm
Emission Max (λem)	~515-525 nm
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.