Description

Bromotrimethylammoniobimane CAS NO 75403-48-4 is a specialized, highly reactive bimane derivative used as a fluorescent labeling reagent for thiol groups. This compound matters for researchers and manufacturers requiring precise, sensitive detection and quantification of sulfhydryl-containing biomolecules like proteins and peptides. It is primarily needed by the life sciences, pharmaceutical R&D, and biotechnology industries for applications in proteomics, enzyme studies, and diagnostic assay development.

Application

• Fluorescent Labeling of Thiol Groups: Primary application for tagging cysteine residues in proteins and peptides for detection and analysis.
• Proteomics Research: Used in gel electrophoresis and chromatography to visualize and quantify protein expression and modifications.
• Enzyme Activity Studies: Employed to monitor enzymatic reactions involving thiol groups or to label active sites.
• Diagnostic Assay Development: Serves as a key reagent in developing fluorescent-based bioassays and kits for clinical or research diagnostics.
• Bioconjugation: Facilitates the creation of fluorescent antibody or protein conjugates for imaging and flow cytometry.
• Pharmaceutical Analysis: Used in quality control and stability testing of biopharmaceuticals to assess protein integrity and aggregation.

Basic Information

Product Name	Bromotrimethylammoniobimane
CAS No.	75403-48-4
Molecular Formula	C12H18BrN3O2
Molecular Weight	316.20 g/mol
Synonyms	qBBr; Bromotrimethylammonio-bimane; Trimethyl(3-bromo-2,7-dimethyl-6-oxo-6H-pyrido[2,3-d]pyrazin-5-yl)ammonium; 3-Bromo-5-(trimethylammonio)-2,7-dimethyl-6H-pyrido[2,3-d]pyrazin-6-one; Thiol-reactive bimane dye; Bimane bromide derivative; qBBr Bimane; Monobromobimane derivative
EINECS	Contact for details

Quality Control

Our Bromotrimethylammoniobimane is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification, to ensure it meets high-purity research and production standards. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may affect stability and performance. For long-term storage, consider desiccation and under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Loss on Drying	≤ 2.0%
Solubility Test	Soluble in DMSO, Acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.