Description

7-Dimethylamino-4-Methylcoumarin-3-Isothiocyanate (Dacitc) is a specialized coumarin derivative functionalized with an isothiocyanate group, making it a highly reactive and valuable labeling reagent. This compound matters for its ability to form stable covalent bonds with primary amines, enabling the fluorescent tagging of biomolecules for sensitive detection and analysis. It is primarily needed by researchers and manufacturers in the life sciences, particularly for developing diagnostic assays, fluorescence microscopy, and protein/antibody conjugation.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to proteins, peptides, and antibodies for fluorescence-based immunoassays (e.g., ELISA, Western Blot).
• Diagnostic Reagent Synthesis: A key intermediate in the production of fluorescent probes and diagnostic kits for clinical and research use.
• Cell and Tissue Imaging: Used as a tagging agent in fluorescence microscopy and histochemistry for visualizing cellular structures.
• Bioconjugation & Chemical Biology: Essential tool for creating bioconjugates in drug discovery and proteomics research.
• Sensor Development: Incorporated into the design of chemosensors and biosensors for detecting specific analytes.
• Pharmaceutical Research: Employed in the development and quality control of biopharmaceuticals.

Basic Information

Product Name	7-Dimethylamino-4-Methylcoumarin-3-Isothiocyanate (Dacitc)
CAS No.	74802-04-3
Molecular Formula	C₁₄H₁₂N₂O₂S
Molecular Weight	272.32 g/mol
Synonyms	Dacitc; 7-(Dimethylamino)-4-methyl-2-oxo-2H-chromene-3-carbonyl isothiocyanate; 7-(Dimethylamino)-4-methylcoumarin-3-isothiocyanate; 4-Methyl-7-(dimethylamino)coumarin-3-isothiocyanate; 3-Isothiocyanato-7-(dimethylamino)-4-methylcoumarin; DMAC-ITC; Fluorescent Isothiocyanate Coumarin Derivative
EINECS	Contact for details

Quality Control

Our 7-Dimethylamino-4-Methylcoumarin-3-Isothiocyanate is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and tests for residual solvents and moisture. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with your research or manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.