Description

5-Oxo-Prolyl-Glycyl-Arginine-4-Nitroanilide is a specialized chromogenic peptide substrate designed for enzymatic activity assays. This compound is critical for researchers and quality control laboratories requiring precise, colorimetric detection of specific protease activity. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic reagent manufacturing sectors for enzyme kinetics studies, inhibitor screening, and assay development.

Application

• Protease Activity Assays: As a chromogenic substrate for the quantitative determination of trypsin, thrombin, and other serine proteases.
• Biochemical Research: Used in enzyme kinetics studies to measure reaction rates and determine kinetic parameters (Km, Vmax).
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for hemostasis and other protease-related pathways.
• Quality Control: Used in QC laboratories for the potency testing of enzyme-based pharmaceuticals and reagents.

Basic Information

Product Name	5-Oxo-Prolyl-Glycyl-Arginine-4-Nitroanilide
CAS No.	74661-31-7
Molecular Formula	C20H30N10O7
Molecular Weight	522.52 g/mol
Synonyms	5-Oxo-Pro-Gly-Arg-pNA; pNA; S-2484 (Substrate); Pyroglutamyl-Glycyl-Arginine 4-Nitroanilide; Chromogenic Substrate S-2484; L-Pyroglutamyl-Glycyl-L-Arginine 4-Nitroanilide Acetate; Trypsin Chromogenic Substrate; H-Pyr-Gly-Arg-pNA
EINECS	Contact for details

Quality Control

Our 5-Oxo-Prolyl-Glycyl-Arginine-4-Nitroanilide is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance (λ max)	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.