Description

Rhodamine B Hydrazide CAS NO 74317-53-6 is a highly reactive hydrazide derivative of the classic Rhodamine B fluorophore. This compound is valued for its role as a critical labeling reagent and precursor in the synthesis of advanced fluorescent probes and sensors. It is primarily sought by researchers and manufacturers in the life sciences, analytical chemistry, and materials science sectors for applications requiring sensitive detection and conjugation chemistry.

Application

• Fluorescent Probe Synthesis: Acts as a key intermediate for creating targeted fluorescent probes for bioimaging and cellular analysis.
• Bioconjugation & Labeling: Used to label proteins, antibodies, and other biomolecules via its reactive hydrazide group, enabling fluorescence-based detection assays.
• Chemical Sensor Development: Incorporated into polymeric or small-molecule frameworks to develop sensors for metal ions, anions, or gases.
• Material Science Research: Employed in the development of fluorescent nanomaterials and smart polymers for diagnostic and sensing applications.
• Dye & Pigment Intermediate: Serves as a specialized precursor in the synthesis of high-performance dyes and pigments.
• Analytical Reagent: Utilized as a derivatization agent in chromatography and spectrometry for the detection of carbonyl compounds.

Basic Information

Product Name	Rhodamine B Hydrazide
CAS No.	74317-53-6
Molecular Formula	C28H31ClN4O2
Molecular Weight	491.03 g/mol
Synonyms	Rhodamine B hydrazide; Rhodamine B acid hydrazide; [9-(2-Carboxyphenyl)-6-diethylamino-3H-xanthen-3-ylidene]-diethylammonium chloride hydrazide; N'-[6-(Diethylamino)-9-(2-carboxyphenyl)-3H-xanthen-3-ylidene]-N-ethylethanaminium chloride hydrazone; RBH; RhB-Hz
EINECS	Contact for details

Quality Control

Our Rhodamine B Hydrazide is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification (NMR, IR), and loss on drying analysis. We provide a Certificate of Analysis (COA) with every shipment, detailing all critical quality parameters to ensure it meets the stringent requirements of research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	Dark red to purple crystalline powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.