Description

Monobromobimane is a highly reactive, thiol-specific fluorescent labeling reagent. Its core value lies in its ability to form stable, fluorescent adducts with thiol groups under mild conditions, enabling sensitive detection and quantification. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors for applications such as protein analysis and redox status determination.

Application

• Fluorescent Labeling of Thiols: Primary application for tagging cysteine residues in proteins, peptides, and other biomolecules for detection via HPLC or fluorescence spectroscopy.
• Proteomics Research: Used in protein structure-function studies, mapping of active sites, and monitoring conformational changes.
• Biomarker Detection: Employed in assays to measure levels of glutathione and other biologically important thiols as indicators of oxidative stress.
• Pharmaceutical Analysis: Aids in the purity testing and characterization of drug substances and products containing thiol groups.
• Diagnostic Kit Development: Serves as a key component in the development of fluorescent-based diagnostic assays and kits.
• Cell Biology Studies: Used to label intracellular thiols for fluorescence microscopy and flow cytometry to study cellular redox environments.

Basic Information

Product Name	Monobromobimane
CAS No.	74235-78-2
Molecular Formula	C11H11BrN2O2
Molecular Weight	283.12 g/mol
Synonyms	mBBr; Bimane, monobromo-; (2-Bromoethyl)trimethylammonium bimane; Thiolite™ MB; 1,5-Diazabicyclo[3.3.0]octa-3,6-diene-2,7-dione, 4-bromomethyl-4-methyl-; 4-Bromomethyl-4-methyl-1,5-diazabicyclo[3.3.0]octa-3,6-diene-2,7-dione
EINECS	Contact for details

Quality Control

Our Monobromobimane is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and analytical applications. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability, consistency, and reliability for your critical workflows.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.