Description

Boc-Val-Pro-Arg-Amc Hcl is a fluorogenic peptide substrate designed for the sensitive detection and study of serine protease activity. This compound matters for researchers and developers requiring precise biochemical tools for enzyme kinetics, inhibitor screening, and diagnostic assay development. It is primarily needed by professionals in pharmaceutical R&D, academic biochemistry laboratories, and diagnostic kit manufacturing for applications in thrombosis research, inflammation studies, and high-throughput screening.

Application

• Protease Activity Assays: A key fluorogenic substrate for measuring the enzymatic activity of thrombin, trypsin, and related serine proteases.
• High-Throughput Screening (HTS): Used in pharmaceutical discovery to identify and characterize novel protease inhibitors for drug development.
• Biochemical Research: Essential for studying enzyme kinetics, specificity, and catalytic mechanisms in academic and industrial research settings.
• Diagnostic Development: Employed in the development of clinical diagnostic assays for conditions related to coagulation and fibrinolysis.
• Quality Control in Biologics: Used to monitor and control the presence of specific protease activities during the production of biopharmaceuticals.

Basic Information

Product Name	Boc-Val-Pro-Arg-Amc Hcl
CAS No.	70375-24-5
Molecular Formula	C30H46N8O7 • HCl
Molecular Weight	667.2 g/mol
Synonyms	Boc-Val-Pro-Arg-AMC hydrochloride; t-Butyloxycarbonyl-Valyl-Prolyl-Arginyl-7-Amido-4-Methylcoumarin hydrochloride; Boc-V-P-R-AMC • HCl; Thrombin Substrate I, Fluorogenic; Boc-VPR-AMC hydrochloride; t-Boc-Val-Pro-Arg-AMC • HCl; 7-Amido-4-methylcoumarin derivative of Boc-Val-Pro-Arg; Fluorogenic peptide substrate for thrombin
EINECS	Contact for details

Quality Control

Our Boc-Val-Pro-Arg-Amc Hcl is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and performance reliability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (Mass Spec)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.