Description

Dodecanoic Acid, 12-[(7-Nitro-2,1,3-Benzoxadiazol-4-Yl)Amino]-, 2,5-Dioxo-1-Pyrrolidinyl Ester is a specialized, fluorescently labeled chemical derivative of lauric acid, designed as a highly reactive active ester for conjugation chemistry. This compound matters for researchers and manufacturers requiring precise, covalent labeling of biomolecules, as the NBD fluorophore provides a sensitive detection handle. It is primarily needed by professionals in biochemical research, pharmaceutical development, and diagnostic assay creation for tagging amines in peptides, proteins, or other nucleophiles.

Application

• Fluorescent Labeling of Biomolecules: Covalent attachment to primary amines (-NH2) on peptides, proteins, or antibodies for detection and imaging studies.
• Bioconjugation & Probe Synthesis: Serving as a key building block for creating custom fluorescent probes and sensor molecules.
• Activity-Based Protein Profiling (ABPP): Used in chemical proteomics to label and identify active enzymes within complex biological samples.
• Diagnostic Assay Development: Incorporation into immunoassays or other diagnostic tests to generate a fluorescent signal.
• Lipid & Fatty Acid Tracing: Fluorescent modification of fatty acid analogs to study lipid metabolism, trafficking, and membrane dynamics.
• Material Science Functionalization: Introducing fluorescent tags onto polymer surfaces or nanoparticles for tracking and characterization.

Basic Information

Product Name	Dodecanoic Acid, 12-[(7-Nitro-2,1,3-Benzoxadiazol-4-Yl)Amino]-, 2,5-Dioxo-1-Pyrrolidinyl Ester
CAS No.	689263-76-1
Molecular Formula	C22H27N5O7
Molecular Weight	473.48 g/mol
Synonyms	NBD-X Lauric Acid Succinimidyl Ester; 12-[(7-Nitro-4-benzofurazanyl)amino]dodecanoic Acid N-Hydroxysuccinimide Ester; Lauric Acid, 12-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-, 2,5-dioxo-1-pyrrolidinyl ester; NBD C12 NHS Ester; NBD-X C12:0 SE; 12-(NBD-amino)dodecanoic acid, succinimidyl ester; NBD-dodecanoic acid, NHS ester; Fluorescent Fatty Acid Labeling Reagent
EINECS	Contact for details

Quality Control

Our production of this high-value biochemical reagent adheres to stringent quality protocols. Each batch undergoes comprehensive analysis, including HPLC for purity verification, spectroscopic identification (NMR, IR), and functional validation of the active ester moiety. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. For custom purity grades or specific application requirements, please contact our technical team.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0 %
Functional Activity	Conjugation efficiency confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.