Description

Histone H4 (2-21) CAS NO 667899-73-2 is a defined peptide fragment derived from the N-terminal tail of the core histone H4, representing amino acid residues 2 through 21. This synthetic peptide is a critical research tool for investigating post-translational modifications, chromatin structure, and epigenetic regulation. It is primarily utilized by researchers and developers in the fields of academic biochemistry, pharmaceutical R&D for epigenetic drug discovery, and diagnostic assay development.

Application

• Biochemical Research: Used as a substrate for studying histone-modifying enzymes such as acetyltransferases, deacetylases, methyltransferases, and kinases.
• Epigenetics & Chromatin Biology: Essential for in vitro binding assays to analyze protein-protein interactions within the nucleosome and chromatin remodeling complexes.
• Antibody Production & Validation: Serves as an immunogen or antigen for generating and characterizing antibodies specific to the H4 N-terminal tail or its modification states.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) assays to identify and validate novel inhibitors or activators of epigenetic targets.
• Diagnostic Development: Used in the development of ELISA and other immunoassays to detect specific histone modifications linked to disease states.
• Peptide Array & Microarray Fabrication: Spotted onto slides to create platforms for profiling antibody specificity or enzyme activity on a proteomic scale.
• Mass Spectrometry Standard: Acts as a reference standard for the quantification of endogenous histone H4 peptides and their modifications via LC-MS/MS.

Basic Information

Product Name	Histone H4 (2-21)
CAS No.	667899-73-2
Molecular Formula	C96H169N39O30
Molecular Weight	2266.6 g/mol
Synonyms	H4 (2-21) Peptide; Histone H4 Residues 2-21; Acetylated Histone H4 (2-21) (potential variant); H4 Tail Peptide (2-21); H4 N-terminal Peptide; Lysine-rich H4 Peptide; Chromatin Peptide H4; Synthetic Histone H4 Fragment; Epigenetic Research Peptide
EINECS	Contact for details

Quality Control

Every batch of Histone H4 (2-21) is produced and analyzed under strict quality protocols to ensure high purity, correct sequence, and batch-to-batch consistency essential for reproducible research. Our products undergo rigorous quality testing, including reversed-phase HPLC and mass spectrometry (MS) for identity and purity confirmation. Certificates of Analysis (COA) detailing purity, molecular weight verification, and solvent content are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Amino Acid Composition	Pass
Peptide Content	≥ 70% (by weight)
Solvent Content (TFA, Water)	Balance

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.