Description

(Lys(Me)3)-Histone H3 (1-21)-Gly-Gly-Lys(Biotinyl) Amide is a chemically modified peptide sequence derived from the N-terminal tail of histone H3, featuring trimethylated lysine residues and a biotin tag. This compound is a critical research tool for studying epigenetic mechanisms, specifically histone methylation and its role in chromatin structure and gene regulation. It is essential for scientists in academic research, pharmaceutical discovery, and diagnostic development who require high-purity, well-characterized biochemical probes.

Application

• Epigenetics Research: Used as a substrate or binding partner in assays to study histone methyltransferase (HMT) or demethylase activity.
• Biomolecular Interaction Studies: The biotin tag enables immobilization on streptavidin-coated surfaces for pull-down assays, ELISA, or surface plasmon resonance (SPR) to investigate protein-protein interactions.
• Antibody Validation & Development: Serves as a critical antigen for testing the specificity of antibodies raised against trimethylated histone H3 (e.g., H3K4me3, H3K9me3, H3K27me3).
• Diagnostic Assay Development: Can be incorporated into platforms for screening and detecting epigenetic modifiers or readers in high-throughput formats.
• Chromatin Biochemistry: Used in in vitro reconstitution experiments to assemble modified nucleosomes and study their functional properties.
• Drug Discovery Screening: Acts as a target in screening campaigns to identify small molecule inhibitors or modulators of epigenetic enzymes.

Basic Information

Product Name	(Lys(Me)3)-Histone H3 (1-21)-Gly-Gly-Lys(Biotinyl) Amide
CAS No.	2022956-73-4
Molecular Formula	C136H238N44O39S
Molecular Weight	3121.7 g/mol
Synonyms	H3 (1-21) Trimethyl Lysine Peptide; Biotinylated Histone H3 (1-21) Trimethyl Lysine; ARTKQTARK(Me3)STGGKAPRKQLA-GG-K(Biotin); H3K4me3/H3K9me3/H3K27me3 Peptide (Biotinylated); Histone H3 N-terminal peptide, trimethylated; Epigenetic Peptide Substrate; Biotin-H3(1-21)K(Me3) Peptide; Lys(Me)3-Histone H3(1-21)-GG-Lys(Biotin)-NH2
EINECS	Contact for details

Quality Control

This high-value biochemical is produced and analyzed under stringent conditions to ensure batch-to-batch consistency and reliability for sensitive research applications. Certificates of Analysis (COA) are provided with each lot, detailing purity, identity, and content. Our quality system adheres to principles relevant for GMP-grade raw materials used in diagnostic and therapeutic development. All analytical data is traceable and available for review.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Amino Acid Analysis	Within ± 10% of theoretical
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, or aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.