Description

5-Methylcytosine is a fundamental nucleobase derivative, playing a critical role in the regulation of gene expression and cellular differentiation. This modified cytosine is essential for epigenetic research, serving as a key marker in DNA methylation studies that underpin advancements in oncology, developmental biology, and diagnostics. It is a vital biochemical reagent for pharmaceutical R&D, molecular biology, and the production of high-purity nucleotide analogs and reference standards.

Application

• Epigenetics & Molecular Biology Research: A critical standard and reagent for studying DNA methylation patterns, gene silencing, and genomic imprinting.
• Pharmaceutical Development: Used in the synthesis of novel nucleoside analogs and prodrugs for antiviral and anticancer therapies.
• Diagnostic Kit Manufacturing: Serves as a key component in the production of kits for detecting methylation status in clinical and research settings.
• Biochemical Synthesis: An important building block for the enzymatic or chemical synthesis of modified oligonucleotides and DNA probes.
• Reference Standard: Used as a certified reference material (CRM) for analytical method development and validation in QC laboratories.
• Life Science Reagents: Incorporated into cell culture media and buffers for specialized stem cell and developmental biology studies.

Basic Information

Product Name	5-Methylcytosine
CAS No.	554-01-8
Molecular Formula	C5H7N3O
Molecular Weight	125.13 g/mol
Synonyms	5-Methylcytosine; 4-Amino-5-methyl-2(1H)-pyrimidinone; 5-MC; m5C; 6-Amino-5-methyl-2-pyrimidinol; 2-Hydroxy-5-methyl-4-aminopyrimidine; Cytosine, 5-methyl-; NSC 13736
EINECS	209-053-6

Quality Control

Our 5-Methylcytosine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and stringent controls for residual solvents and impurities. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with your research and production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed immediately after use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Assay (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.