Description

(Lys(Ac)5.8.12.16)-Histone H4 (1-25)-Gly-Ser-Gly-Ser-Lys(Biotinyl) is a precisely engineered, chemically modified peptide fragment derived from the N-terminal tail of Histone H4. This compound is critical for advanced epigenetic research, serving as a high-purity, biotinylated substrate to study site-specific lysine acetylation and its role in chromatin structure and gene regulation. It is an essential reagent for biochemists and molecular biologists working in academic research, pharmaceutical discovery, and diagnostic assay development, particularly for pull-down assays, protein-protein interaction studies, and enzyme activity profiling.

Application

• Epigenetics Research: As a defined substrate for studying histone acetyltransferase (HAT) and deacetylase (HDAC) enzyme activity and specificity.
• Biochemical Assay Development: Utilized in high-throughput screening (HTS) platforms to identify and characterize novel epigenetic modulators and drug candidates.
• Chromatin Interaction Studies: The biotin tag enables efficient immobilization for pull-down assays to investigate binding partners like bromodomain-containing proteins.
• Diagnostic Tool Manufacturing: Incorporated into ELISA or other immunoassay kits for the detection and quantification of specific epigenetic markers.
• Antibody Validation: Serves as a critical antigen for generating and validating antibodies specific to multi-acetylated Histone H4.
• Mass Spectrometry Standards: Used as an internal reference standard in quantitative proteomics and mass spectrometry-based epigenetic analyses.

Basic Information

Item	Detail
Product Name	(Lys(Ac)5.8.12.16)-Histone H4 (1-25)-Gly-Ser-Gly-Ser-Lys(Biotinyl)
CAS No.	2022956-65-4
Molecular Formula	C173H295N55O53S
Molecular Weight	~4032.6 g/mol
Synonyms	Acetylated and Biotinylated H4 (1-25) Peptide; H4K5acK8acK12acK16ac-biotin; Multi-acetylated Histone H4 (1-25) Biotin Conjugate; Biotinyl-Lys(Ac)-Histone H4 Fragment; Epigenetic Research Peptide H4; Ac-H4(1-25)-biotin; Histone H4 N-terminal tail substrate; Biotinylated tetra-acetyl H4 peptide
EINECS	Contact for details

Quality Control

Every batch of our (Lys(Ac)5.8.12.16)-Histone H4 (1-25)-Gly-Ser-Gly-Ser-Lys(Biotinyl) is synthesized and purified under stringent conditions to ensure the highest standards of identity, purity, and biological activity. Quality is verified by a comprehensive battery of analytical techniques including HPLC and mass spectrometry. Certificates of Analysis (COA) detailing purity, molecular weight confirmation, and modification verification are provided with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. Aliquot into single-use quantities to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Molecular weight confirmed by ESI-MS or MALDI-TOF
Purity (HPLC)	≥ 95%
Amino Acid Analysis	Complies with theoretical composition
Water Content (KF)	≤ 5.0%
Acetic Acid Content (HPLC)	≤ 10.0%
Biological Activity	Functional in standard enzyme assay (data provided)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.