Description

SMCC Crosslinker is a heterobifunctional, amine-to-sulfhydryl crosslinking reagent featuring an NHS ester and a maleimide group. This compound is essential for creating stable, site-specific conjugates between proteins, peptides, and other biomolecules, enabling advanced research and therapeutic development. It is a critical tool for scientists and manufacturers in the biopharmaceutical, diagnostic, and life science research sectors who require precise and reliable conjugation chemistry.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic drugs to monoclonal antibodies via cysteine residues.
• Protein-Protein Conjugation: Creation of bispecific antibodies, fusion proteins, or protein complexes for therapeutic and diagnostic applications.
• Enzyme Immobilization: Covalent attachment of enzymes to solid supports (e.g., beads, plates, sensors) for biocatalysis and assay development.
• Peptide Labeling: Introduction of fluorescent dyes, biotin, or other probes onto peptides containing free thiol groups.
• Nanoparticle Functionalization: Conjugation of targeting ligands (e.g., antibodies, peptides) to the surface of nanoparticles for targeted drug delivery.
• Diagnostic Assay Development: Linking detection enzymes or signal molecules to antibodies or antigens in ELISA and lateral flow assays.

Basic Information

Product Name	SMCC Crosslinker
CAS No.	64987-85-5
Molecular Formula	C16H20N2O8
Molecular Weight	368.34 g/mol
Synonyms	Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate; SMCC; 4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester; NHS-PEG4-Maleimide (related); Sulfo-SMCC (sulfonated analogue); SM(PEG)n linkers (related class); Heterobifunctional crosslinker; Amine-to-Thiol crosslinker
EINECS	Contact for details

Quality Control

Our SMCC Crosslinker is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and functional group analysis, to ensure it meets the high standards required for sensitive bioconjugation applications. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming compliance with our rigorous specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, inert environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile
Functional Group Assay (NHS Ester)	≥ 90% of theoretical
Functional Group Assay (Maleimide)	≥ 90% of theoretical

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.