Description

N7-Methyl-Guanosine-5'-Triphosphate-5'-Adenosine is a chemically modified dinucleotide cap analog, a critical reagent in molecular biology research. Its primary value lies in its ability to enhance the stability and translational efficiency of in vitro transcribed (IVT) mRNA, which is essential for applications in mRNA therapeutics and vaccine development. This high-purity compound is specifically needed by researchers and manufacturers in the biotechnology and pharmaceutical industries focused on advancing mRNA-based technologies, synthetic biology, and epigenetics studies.

Application

• mRNA Synthesis & Capping: Used as a cap analog in in vitro transcription reactions to produce 5'-capped mRNA with superior co-transcriptional capping efficiency, crucial for therapeutic mRNA production.
• Vaccine Development: A key raw material in the research and manufacturing of mRNA vaccines, where proper 5' capping is vital for immune recognition and protein expression.
• Gene Therapy Research: Employed in the synthesis of mRNA constructs for gene editing and protein replacement therapy studies.
• Structural Biology Studies: Serves as a substrate or inhibitor for studying the structure and function of mRNA cap-binding proteins and methyltransferases.
• Biochemical Assay Development: Used as a standard or critical reagent in assays designed to screen for cap-binding compounds or to measure enzymatic activity related to mRNA processing.

Basic Information

Product Name	N7-Methyl-Guanosine-5'-Triphosphate-5'-Adenosine
CAS No.	62828-63-1
Molecular Formula	C21H29N10O17P3
Molecular Weight	834.43 g/mol
Synonyms	m7G(5')ppp(5')A; m7GpppA; N7-Methyl-GTP-5'-Adenosine; Cap 0 Analog (m7GpppA); 7-Methylguanosine-5'-triphospho-5'-adenosine; Anti-Reverse Cap Analog (ARCA) variant; Dinucleotide Cap Analog; mRNA Cap 0 Structure
EINECS	Contact for details

Quality Control

Our N7-Methyl-Guanosine-5'-Triphosphate-5'-Adenosine is manufactured under stringent conditions to ensure the high purity and consistency required for sensitive life science applications. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation via mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided and can be tailored to meet specific research or cGMP guidelines for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or suitable aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.