Description

Lissamine Rhodamine B Sulfonyl Chloride is a highly reactive, fluorescent labeling reagent used for the derivatization of amines, amino acids, and proteins. This compound is critical for creating stable, fluorescent conjugates with excellent photostability and high quantum yield, enabling sensitive detection and analysis. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, proteomics, diagnostics, and fluorescence microscopy for tagging biomolecules.

Application

• Fluorescent Labeling of Biomolecules: Primary reagent for covalently attaching a bright red-orange fluorescent tag to amines in proteins, peptides, antibodies, and oligonucleotides.
• Proteomics and Bioanalytical Research: Used in gel electrophoresis, Western blotting, and HPLC for the detection and quantification of low-abundance proteins.
• Diagnostic Assay Development: Essential for creating fluorescent probes and tracers used in immunoassays (e.g., ELISA, lateral flow tests) and in vitro diagnostics (IVD).
• Cell and Tissue Imaging: Enables the preparation of fluorescent dyes for staining cellular components in fluorescence microscopy and flow cytometry applications.
• Pharmaceutical Analysis: Employed as a derivatization agent in HPLC and LC-MS methods for the sensitive analysis of amine-containing pharmaceuticals.
• Material Science: Used to functionalize surfaces and nanomaterials with fluorescent markers for tracking and sensing applications.

Basic Information

Product Name	Lissamine Rhodamine B Sulfonyl Chloride
CAS No.	62796-29-6
Molecular Formula	C27H29ClN2O7S2
Molecular Weight	593.11 g/mol
Synonyms	Lissamine Rhodamine B Sulfonyl Chloride; Sulforhodamine B Sulfonyl Chloride; Lissamine™ Rhodamine B Sulfonyl Chloride; Rhodamine B Sulfonyl Chloride; Acid Red 52 Sulfonyl Chloride; SRB Sulfonyl Chloride; 5-(4-Chlorosulfonylphenylazo)-6-(diethylamino)-3H-xanthen-3-one; LRSCl; Contact for EINECS details.

Quality Control

Our Lissamine Rhodamine B Sulfonyl Chloride is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain reactivity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in anhydrous DMF, DMSO, acetonitrile
Functional Suitability	Conforms for labeling applications

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.