Description

Hiv Protease Nonapeptide Substrate CAS NO 61970-47-6 is a specialized synthetic peptide sequence designed as a selective substrate for the enzymatic activity of HIV-1 protease. This compound is critical for biochemical research and pharmaceutical development, enabling the study of protease kinetics, inhibitor screening, and assay validation. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in antiviral drug discovery and development.

Application

• Enzyme Kinetics Studies: Used as a specific substrate to determine the kinetic parameters (Km, Vmax) of HIV-1 protease.
• High-Throughput Screening (HTS): Essential for screening libraries of compounds to identify and characterize potential HIV protease inhibitors in drug discovery campaigns.
• Diagnostic Assay Development: Serves as a key component in the development of colorimetric, fluorometric, or other assay formats for protease activity detection.
• Mechanism of Action Research: Employed in studies to understand the cleavage specificity and catalytic mechanism of HIV protease.
• Quality Control for Inhibitors: Used as a reference substrate to test the potency and efficacy of developed protease inhibitor candidates.
• Biochemical Reagent: A vital tool for basic virology and biochemistry research focused on the HIV replication cycle.

Basic Information

Item	Detail
Product Name	Hiv Protease Nonapeptide Substrate
CAS No.	61970-47-6
Molecular Formula	C₄₆H₆₈N₁₂O₁₂
Molecular Weight	981.11 g/mol
Synonyms	HIV-1 Protease Substrate; HIV Protease Substrate (Nonapeptide); Hiv Protease Substrate (9-mer); Acetyl-Thr-Ile-Nle-[CH2-NH]-Nle-Gln-Arg-NH2; Ac-Thr-Ile-Nle-ψ[CH2NH]-Nle-Gln-Arg-NH2; Reduced Peptide Bond Inhibitor Substrate; HIV PR Substrate; Protease Cleavage Site Substrate
EINECS	Contact for details

Quality Control

Our Hiv Protease Nonapeptide Substrate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is characterized using advanced analytical techniques including HPLC and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 80%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Biological Activity	Cleaved by HIV-1 protease (activity confirmed by assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.