Description

n-[4-(Aminomethyl)Benzyl]Rhodamine 6G-Amide Bis(Trifluoroacetate) is a high-purity, functionalized derivative of the Rhodamine 6G fluorophore, designed for advanced bioconjugation and labeling applications. Its primary value lies in its reactive primary amine group, which enables covalent attachment to biomolecules like proteins, peptides, and nucleic acids for creating highly specific fluorescent probes. This compound is essential for researchers and manufacturers in the fields of life sciences, diagnostics, and chemical biology who require precise and stable fluorescent tags for imaging, flow cytometry, and assay development.

Application

• Fluorescent Probe Synthesis: Covalent labeling of antibodies, proteins, and oligonucleotides for use in immunofluorescence (IF), fluorescence in situ hybridization (FISH), and other detection assays.
• Bioimaging & Microscopy: Development of cell-permeable or targeted fluorescent dyes for live-cell imaging, confocal microscopy, and super-resolution imaging techniques.
• Flow Cytometry: Conjugation to detection antibodies or ligands for high-sensitivity multiparameter flow cytometry analysis.
• Diagnostic Assay Development: Key component in the manufacture of lateral flow assays, ELISA kits, and other point-of-care diagnostic tools requiring bright, photostable signals.
• Chemical Biology Research: Tool for studying protein-protein interactions, enzyme activity, and cellular trafficking through site-specific labeling strategies.
• Material Science: Potential use in the development of fluorescent sensors, smart materials, and photonic devices due to its excellent photophysical properties.

Basic Information

Product Name	n-[4-(Aminomethyl)Benzyl]Rhodamine 6G-Amide Bis(Trifluoroacetate)
CAS No.	591742-78-8
Molecular Formula	C36H33F6N5O5
Molecular Weight	729.67 g/mol
Synonyms	Rhodamine 6G Amine Derivative; 5-(and-6)-((N-(4-Aminobenzyl)amino)carbonyl) Rhodamine 6G Bis(trifluoroacetate); X-Rhodamine Amine; Rhodamine 6G, N-[4-(aminomethyl)benzyl]amide, bis(trifluoroacetate); Reactive Rhodamine 6G; Amino-reactive Rhodamine 6G; 6-TAMRA amine derivative; 5/6-TAMRA, SE alternative
EINECS	Contact for details

Quality Control

Every batch of n-[4-(Aminomethyl)Benzyl]Rhodamine 6G-Amide Bis(Trifluoroacetate) is manufactured under strict quality management systems. Our products undergo rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure identity, purity, and performance consistency suitable for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the primary container in a sealed secondary container with an appropriate desiccant. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Red to dark red solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.