Description

4-(Glycyl-L-Phenylalanylamido)-2,2,6,6-Tetramethylpiperidinyl-1-Oxy is a specialized spin-labeled peptide derivative, integrating a stable nitroxide radical (TEMPO) with a dipeptide backbone. This compound matters as a critical tool in advanced research, serving as a paramagnetic probe for investigating protein structure, dynamics, and interactions via techniques like Electron Paramagnetic Resonance (EPR) spectroscopy and site-directed spin labeling (SDSL). Researchers in structural biology, biophysics, and pharmaceutical development need it to gain molecular-level insights into complex biological systems, enabling the study of conformational changes, binding sites, and aggregation processes that are difficult to observe with other methods.

Application

• Site-Directed Spin Labeling (SDSL): Covalent attachment to specific sites in proteins or peptides for EPR spectroscopy studies.
• Protein Structure & Dynamics Analysis: Probing local conformational changes, flexibility, and distances within biomolecules.
• Membrane Protein Research: Investigating the topology and environmental changes of proteins within lipid bilayers.
• Drug Discovery & Development: Studying ligand-protein interactions and binding kinetics to support rational drug design.
• Biophysical Characterization: Used in EPR and NMR spectroscopy to determine structural parameters and monitor folding/unfolding events.
• Oxidative Stress & Radical Research: Acting as a stable radical source or scavenger in model systems studying redox biology.
• Material Science: Functionalization of surfaces or polymers to create smart materials with paramagnetic properties.

Basic Information

Product Name	4-(Glycyl-L-Phenylalanylamido)-2,2,6,6-Tetramethylpiperidinyl-1-Oxy
CAS No.	59995-91-4
Molecular Formula	C21H31N5O4
Molecular Weight	417.50 g/mol
Synonyms	Gly-Phe-TEMPO; 4-(Glycyl-L-phenylalanylamido)-TEMPO; TEMPO-Gly-Phe-NH2; 2,2,6,6-Tetramethylpiperidin-1-oxyl-4-amino-4-carboxylic acid glycyl-L-phenylalanine amide; Spin-labeled Gly-Phe dipeptide; SL-Gly-Phe; N-(2,2,6,6-Tetramethyl-1-oxyl-piperidin-4-yl)-N'-(glycyl-L-phenylalanyl)urea (alternative structural representation); 4-Isocyanato-2,2,6,6-tetramethylpiperidine-1-oxyl glycyl-L-phenylalanine amide
EINECS	Contact for details

Quality Control

Our 4-(Glycyl-L-Phenylalanylamido)-2,2,6,6-Tetramethylpiperidinyl-1-Oxy is produced under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, EPR for radical content verification, and spectroscopic methods for structural confirmation. Certificates of Analysis (COA) detailing purity, assay, and specific test results are provided to guarantee compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This compound is easily oxidized and light-sensitive; allow it to reach room temperature in a desiccator before opening to minimize exposure to moisture and oxygen. For short-term use, storage at 2-8°C in the dark is acceptable.

Specification

Item	Specification
Appearance	Orange to red powder or crystals
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95% (Area %)
Assay (EPR)	≥ 90% radical content
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.