Description

Poly(Dg).Poly(Rc) CAS NO 54482-00-7 is a synthetic polynucleotide complex, specifically a double-stranded RNA (dsRNA) analog formed by the hybridization of polydeoxyguanylic acid and polyribocytidylic acid strands. This compound is a critical research tool for studying nucleic acid interactions, immune system activation, and antiviral mechanisms. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical developers focused on immunology, virology, and novel therapeutic platforms.

Application

• Immunological Research: Used as a model dsRNA to study the activation of pattern recognition receptors (PRRs) like TLR3, RIG-I, and MDA5, simulating viral infection responses.
• Vaccine Adjuvant Development: Investigated for its potential to enhance immune responses in vaccine formulations by stimulating innate immunity.
• Antiviral Agent Screening: Serves as a standard or challenge agent in in vitro assays to evaluate the efficacy of new antiviral compounds.
• Gene Expression Studies: Employed to induce interferon and cytokine production in cell culture models, facilitating the study of downstream signaling pathways.
• Biochemical Standard: Acts as a reference material in spectroscopic studies (UV, CD) and chromatography for nucleic acid characterization.
• Therapeutic Nucleic Acid Research: Provides a structural model for designing and testing stable, immunomodulatory RNA-based therapeutics.

Basic Information

Product Name	Poly(Dg).Poly(Rc) CAS NO 54482-00-7
CAS No.	54482-00-7
Molecular Formula	(C10H12N5O7P)n•(C9H12N3O7P)n
Molecular Weight	Variable (Polymer)
Synonyms	Poly(deoxyguanylic acid)-poly(ribocytidylic acid); Poly(dG)-Poly(rC); Poly(dG):Poly(rC); dG:rC duplex; Poly(dG-dG)-Poly(rC-rC) (alternate notation); Double-stranded RNA analog; Synthetic dsRNA; Immunostimulatory RNA
EINECS	Contact for details

Quality Control

Our Poly(Dg).Poly(Rc) is produced and tested under stringent quality protocols to ensure batch-to-batch consistency and research reliability. Each lot is characterized for identity, purity, and biophysical properties. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications such as base composition, strand length, duplex formation, and absence of nucleases. We adhere to ISO 9001 quality management principles, and custom synthesis to meet specific research-grade requirements is available.

Storage

Preserve in a tightly closed container, protected from light. Store frozen at -20°C or below as a lyophilized powder or in a buffered aqueous solution. For solutions, aliquot to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed under anhydrous conditions or in a desiccator.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or fibrous solid
Identification (UV-Vis)	Characteristic absorption spectrum for dsRNA
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 95% (as duplex)
Strand Length (SEC/GPC)	Specified range (e.g., 200-1000 base pairs) available per grade
Base Ratio (dG:rC)	~1:1 (by HPLC analysis after digestion)
Duplex Formation (CD/Tm)	Confirmed by circular dichroism; Tm provided
Endotoxin Level (LAL)	< 10 EU/mg (for cell culture grades)
Solubility	Soluble in TE buffer or nuclease-free water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.