Description

5'-Mercuriuridine Triphosphate is a modified nucleotide triphosphate where a mercury atom is incorporated into the uridine base. This compound is a critical reagent in specialized biochemical research and molecular biology applications, particularly for nucleic acid labeling and structural studies. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical R&D departments working on nucleic acid probes, cross-linking experiments, and heavy-atom derivatization for X-ray crystallography.

Application

• Nucleic Acid Labeling & Probe Synthesis: Acts as a heavy-atom label for the preparation of mercurated nucleic acid probes used in hybridization assays and electron microscopy.
• X-ray Crystallography Studies: Serves as a heavy-atom derivative for phase determination in the structural analysis of nucleic acids and nucleic acid-protein complexes.
• Biochemical Cross-linking: Used in studies investigating nucleic acid structure and protein-nucleic acid interactions via mercury-mediated cross-linking.
• Enzymology Research: Employed as a substrate or inhibitor in studies of DNA/RNA polymerase mechanisms and fidelity.
• Diagnostic Assay Development: A key component in developing specialized diagnostic tests that rely on mercurated nucleotide incorporation.
• Spectroscopic Analysis: Utilized in various spectroscopic techniques (e.g., NMR) as a paramagnetic probe to study nucleic acid conformation and dynamics.

Basic Information

Item	Detail
Product Name	5'-Mercuriuridine Triphosphate
CAS No.	53293-39-3
Molecular Formula	C9H13HgN2O14P3
Molecular Weight	Contact for details
Synonyms	5-Mercuri-UTP; Mercurated UTP; 5-Hg-UTP; 5-(Mercuri)uridine-5'-triphosphate; Uridine 5'-(tetrahydrogen triphosphate), 5-mercuri derivative; 5-Mercurouridine triphosphate; Hg-UTP
EINECS	Contact for details

Quality Control

Our 5'-Mercuriuridine Triphosphate is produced under stringent quality protocols to ensure batch-to-batch consistency and high purity, essential for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to rigorous analytical standards, utilizing techniques such as HPLC, NMR, and mass spectrometry for verification.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Mercury Content	Confirmatory
Water Content (KF)	< 2.0%
Solubility	Soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.