Description

6-Fam-C6-Oh is a high-purity fluorescent labeling reagent, specifically a carboxyfluorescein derivative with a C6 spacer and a terminal hydroxyl group. This compound is essential for researchers and manufacturers requiring precise, stable fluorescent tagging of biomolecules, peptides, and other substrates. It is primarily utilized in life science research, diagnostic assay development, and pharmaceutical discovery for applications such as fluorescence resonance energy transfer (FRET), fluorescence microscopy, and flow cytometry.

Application

• Fluorescent Labeling of Biomolecules: Covalent attachment to peptides, proteins, oligonucleotides, and antibodies for detection and quantification.
• FRET (Fluorescence Resonance Energy Transfer) Studies: Serving as a donor or acceptor fluorophore in proximity-based assays to study molecular interactions.
• Diagnostic Assay Development: Key component in the synthesis of fluorescent probes and conjugates for immunoassays (e.g., ELISA) and in-situ hybridization (FISH).
• Cell Imaging and Microscopy: Used to label cellular components for real-time visualization and tracking in live or fixed cells.
• Flow Cytometry: Conjugation to antibodies or other ligands for the detection and sorting of specific cell populations.
• Drug Discovery & High-Throughput Screening (HTS): Tagging of small molecules or biomolecular targets in activity and binding assays.
• Material Science: Incorporation into polymers or surfaces to create fluorescent materials for sensing applications.

Basic Information

Product Name	6-Fam-C6-Oh
CAS No.	467456-19-5
Molecular Formula	C31H31NO9
Molecular Weight	561.58 g/mol
Synonyms	6-Carboxyfluorescein-C6-OH; 5(6)-Carboxyfluorescein hexanoic acid; FAM-C6-acid; 6-FAM C6 acid; 6-FAM-C6-Acid; 6-FAM-C6-COOH; 6-FAM-ε-Ahx-OH; 5(6)-FAM, X hexanoic acid
EINECS	Contact for details

Quality Control

Our 6-Fam-C6-Oh is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry for fluorescence properties. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics is provided with every shipment to ensure it meets the stringent requirements of research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	492 - 498 nm
Emission Maximum	515 - 525 nm
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.