Description

7-Methoxycoumarin-4-Acetic Acid n-Succinimidyl Ester is a highly reactive, amine-reactive fluorescent labeling reagent. This compound is critical for researchers and manufacturers who require a stable, efficient method to conjugate fluorophores to proteins, peptides, antibodies, and other biomolecules via primary amine groups. It is primarily needed by the life sciences, diagnostic, and pharmaceutical development sectors for applications in fluorescence microscopy, flow cytometry, immunoassays, and biochemical research.

Application

• Fluorescent Labeling of Biomolecules: Primary reagent for covalently attaching a bright, blue-fluorescent coumarin tag to proteins, antibodies, peptides, and oligonucleotides for detection and analysis.
• Diagnostic Assay Development: Used in the synthesis of fluorescent probes and conjugates for ELISA, lateral flow assays, and other immunoassay platforms.
• Cellular Imaging and Microscopy: Enables the preparation of fluorescently-labeled ligands, tracers, and substrates for live-cell and fixed-cell imaging studies.
• Flow Cytometry Reagents: Key intermediate in manufacturing fluorescent antibody conjugates and other detection reagents for flow cytometry applications.
• Bioconjugation and Probe Synthesis: Serves as a versatile building block for creating custom fluorescent sensors, enzyme substrates, and molecular probes.
• Pharmaceutical Research: Utilized in drug discovery for labeling target molecules to study binding, localization, and pharmacokinetics.

Basic Information

Product Name	7-Methoxycoumarin-4-Acetic Acid n-Succinimidyl Ester
CAS No.	359436-89-8
Molecular Formula	C16H13NO7
Molecular Weight	331.28 g/mol
Synonyms	7-Methoxycoumarin-4-acetic Acid N-Hydroxysuccinimide Ester; 4-Methylumbelliferone-4-Acetic Acid NHS Ester; 7-Methoxy-4-coumarinylacetic Acid N-Succinimidyl Ester; 7-Methoxy-4-coumarinylacetic Acid NHS Ester; 4-MUMA NHS Ester; 7-Methoxycoumarin-4-ylacetic Acid Succinimidyl Ester; 7-Methoxy-4-methylcoumarin-4-acetic Acid N-Hydroxysuccinimide Ester
EINECS	Contact for details

Quality Control

Our 7-Methoxycoumarin-4-Acetic Acid n-Succinimidyl Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Off-white to pale yellow crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Assay	≥ 97.0% (on anhydrous basis)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.