Description

2'-o-Me-2-Thio-U-3'-Phos Phoramidite is a chemically modified phosphoramidite monomer, a critical building block in the solid-phase synthesis of oligonucleotides. Its value lies in enabling the precise incorporation of a 2'-O-methyl-2-thiouridine modification, which significantly enhances the nuclease resistance, thermal stability, and binding affinity of therapeutic RNA molecules. This high-purity reagent is essential for researchers and manufacturers in the fields of antisense oligonucleotide (ASO) development, siRNA therapeutics, and diagnostic probe synthesis, where structural control is paramount for efficacy and specificity.

Application

• Synthesis of Modified Antisense Oligonucleotides (ASOs): For the development of next-generation therapeutics with improved pharmacokinetic profiles and target engagement.
• siRNA and miRNA Construct Manufacturing: To introduce stabilizing 2'-O-methyl and 2-thio modifications, crucial for in vivo stability and potency of RNAi-based drugs.
• Diagnostic Probe and Primer Design: For creating highly specific and stable nucleic acid probes used in PCR, FISH, and other molecular diagnostics.
• Structural Biology Studies: As a tool nucleotide to investigate RNA structure-function relationships and protein-RNA interactions.
• Aptamer Development: For generating nuclease-resistant aptamers with potential therapeutic and diagnostic applications.
• Synthesis of Splice-Switching Oligonucleotides (SSOs): To create modified SSOs with enhanced resistance to degradation for treating genetic disorders.

Basic Information

Product Name	2'-o-Me-2-Thio-U-3'-Phos Phoramidite
CAS No.	302918-84-9
Molecular Formula	C38H49N4O7PSSi
Molecular Weight	757.0 g/mol (approx.)
Synonyms	2'-O-Methyl-2-thiouridine 3'-CE Phosphoramidite; 2'-O-Methyl-2-thio-uridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyl-2-thiouridine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 2'-O-Me-2-S-U Phosphoramidite; 2'-OMe-2-thioU CE Phosphoramidite; DMT-2'-O-methyl-2-thiouridine-3'-CE phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of 2'-o-Me-2-Thio-U-3'-Phos Phoramidite is manufactured and tested under strict quality systems to ensure it meets the high-purity standards required for oligonucleotide synthesis. Our quality commitment includes comprehensive analytical testing by HPLC, 31P NMR, and 1H NMR to verify identity, purity, and stability. Certificates of Analysis (COA) with full traceability are provided for each lot, supporting development under GMP and ISO 9001 guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere (argon or nitrogen). This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC, 260 nm)	≥ 97.0%
Moisture (Karl Fischer)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.