Description

Fluorescamine is a highly sensitive, non-fluorescent reagent that reacts rapidly with primary amines to form stable, highly fluorescent products. This property makes it an indispensable tool for the detection and quantification of amino acids, peptides, proteins, and other primary amines in solution. It is extensively utilized in biochemical research, pharmaceutical quality control, and analytical chemistry. Fluorescamine CAS NO 38183-12-9 offers a critical advantage for sensitive assays where background fluorescence must be minimized.

Application

• Biochemical Assays: Sensitive detection and quantification of primary amines in amino acid analysis, peptide sequencing, and protein concentration determination.
• Pharmaceutical Analysis: Used in purity testing of drug substances, monitoring of reaction completion in peptide synthesis, and analysis of aminoglycoside antibiotics.
• Proteomics & Life Science Research: Fluorescent labeling of proteins for gel electrophoresis, blotting techniques, and cell biology studies to track biomolecules.
• HPLC & LC-MS Detection: Acts as a pre-column or post-column derivatization agent to enable highly sensitive fluorescence detection of amine-containing compounds.
• Food & Beverage Testing: Analysis of amino acid content, protein hydrolysates, and detection of biogenic amines as quality and safety markers.
• Diagnostic Reagent Manufacturing: A key component in formulating diagnostic kits for clinical chemistry, particularly for enzymes like proteases.

Basic Information

Product Name	Fluorescamine
CAS No.	38183-12-9
Molecular Formula	C17H10O4
Molecular Weight	278.26 g/mol
Synonyms	4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione; Fluoram; Fluram; 4-Phenylspiro[furan-2(3H),1'(3'H)-isobenzofuran]-3,3'-dione; Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(dimethylamino)-; (Reagent for primary amines)
EINECS	253-830-1

Quality Control

Our Fluorescamine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, identity confirmation by IR spectroscopy, and loss on drying. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all specifications and test results to ensure compliance with your application requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	Off-white to pale yellow crystalline powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0 %
Fluorescence Suitability	Passes test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.