Description

Thymidylyl-(3'-5')-Thymidine Methyl Phosphotriester is a chemically synthesized dinucleotide derivative, specifically a methyl phosphotriester analog of thymidylyl-thymidine. This compound is of significant value as a key intermediate and model compound in oligonucleotide synthesis research and development. It is primarily needed by researchers and manufacturers in the fields of pharmaceutical development, molecular biology, and diagnostic reagent production, where it aids in studying nucleic acid chemistry, stability, and novel synthetic methodologies.

Application

• Research & Development in Oligonucleotide Synthesis: Serves as a critical model compound and intermediate for developing and optimizing phosphotriester and other solid-phase synthesis techniques.
• Pharmaceutical Development: Used in the research phase for creating antisense oligonucleotides, siRNA, and other therapeutic nucleic acid analogs.
• Biochemical Research: Acts as a substrate or standard in enzymatic studies involving nucleases, polymerases, and other nucleic acid-interacting enzymes.
• Diagnostic Reagent Manufacturing: Employed in the synthesis of probes and primers for molecular diagnostic assays.
• Academic Research: Utilized in university and institutional labs for fundamental studies on DNA structure, stability, and chemical modification.
• Quality Control & Standardization: Functions as a reference standard for the analysis and quality assurance of synthetic oligonucleotides.

Basic Information

Product Name	Thymidylyl-(3'-5')-Thymidine Methyl Phosphotriester
CAS No.	35002-94-9
Molecular Formula	C21H28N4O14P
Molecular Weight	600.44 g/mol
Synonyms	dTpT Methyl Phosphotriester; Thymidylyl(3'→5')thymidine Methyl Phosphotriester; TpT Methyl Ester; 5'-O-[Hydroxy(5'-O-[(2R,3S,5R)-3-Hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)oxolan-2-yl]methoxy)phosphoryl]thymidine; Methyl P-TpT; d(TPT) Methyl Phosphotriester; Dinucleotide TpT Methyl Phosphotriester; Thymidyl Thymidine Methyl Phosphotriester
EINECS	Contact for details

Quality Control

Our Thymidylyl-(3'-5')-Thymidine Methyl Phosphotriester is produced under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to stringent internal quality standards to meet the demands of pharmaceutical R&D and analytical reference markets.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, Methanol; Slightly soluble in Water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.