Description

5-Bromo-2'-Deoxycytidine 5'-Triphosphate Sodium is a modified nucleotide triphosphate analog, a critical building block for advanced molecular biology and diagnostic applications. Its core value lies in enabling site-specific incorporation of brominated cytidine into DNA, which is essential for techniques like X-ray crystallography, cross-linking studies, and next-generation sequencing. This high-purity biochemical is primarily needed by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers developing cutting-edge genomic tools and therapeutic oligonucleotides.

Application

• Next-Generation Sequencing (NGS): Used as a substrate for DNA polymerases in sequencing-by-synthesis platforms to study epigenetic modifications or for specialized sequencing applications.
• X-ray Crystallography & Structural Biology: The bromine atom acts as a heavy atom for phasing, facilitating the determination of three-dimensional nucleic acid structures.
• Polymerase Chain Reaction (PCR): Employed in the synthesis of bromine-labeled DNA probes for sensitive detection in assays like qPCR.
• Site-Directed Mutagenesis & Cross-linking Studies: Incorporated into oligonucleotides to create specific cross-linking sites or to study protein-DNA interactions.
• Diagnostic Assay Development: A key component in the manufacture of probes for clinical diagnostics, including fluorescence in situ hybridization (FISH) and microarray technologies.
• Antiviral & Anticancer Research: Serves as a precursor or analog in the development of nucleoside-based therapeutic agents.

Basic Information

Product Name	5-Bromo-2'-Deoxycytidine 5'-Triphosphate Sodium
CAS No.	30419-11-5
Molecular Formula	C9H13BrN3O13P3•Nax
Molecular Weight	Contact for details
Synonyms	5-Bromo-dCTP Sodium Salt; BrdCTP; 5-Bromo-2'-deoxycytidine-5'-triphosphate trisodium salt; 5-Bromodeoxycytidine triphosphate; 5-Br-dCTP; Br-2'-deoxycytidine-5'-triphosphate; 1-(2-Deoxy-β-D-erythro-pentofuranosyl)-5-bromocytosine 5'-(trihydrogen triphosphate) sodium salt
EINECS	Contact for details

Quality Control

Our 5-Bromo-2'-Deoxycytidine 5'-Triphosphate Sodium is manufactured under stringent quality systems to ensure batch-to-batch consistency and reliability for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards required for molecular biology. A Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate. Aliquot into single-use vials to avoid repeated freeze-thaw cycles and maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Solubility	Soluble in water
pH (1% Solution)	6.0 - 8.0
Water Content (KF)	≤ 5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.