Description

Guanylyl-(5'-3')-Cytidylyl-(5'-3')-Adenosine is a defined trinucleotide sequence, commonly known as GpCpA, with the CAS number 3393-25-7. This compound serves as a critical research-grade biochemical building block for studying RNA structure, function, and enzymatic processes. It is essential for scientists and manufacturers in pharmaceutical research, molecular biology, and oligonucleotide synthesis, where precise nucleotide sequences are required for developing diagnostics and therapeutic agents.

Application

• Biochemical Research: Used as a substrate or model compound in enzymatic studies of RNA polymerases, nucleases, and other RNA-modifying enzymes.
• Oligonucleotide Synthesis: Serves as a key intermediate or reference standard in the solid-phase synthesis of custom RNA sequences.
• Structural Biology: Employed in X-ray crystallography and NMR spectroscopy to investigate RNA three-dimensional structure and folding dynamics.
• Diagnostic Development: Acts as a component in the design and calibration of assays for viral RNA detection and genetic analysis.
• Therapeutic Research: Utilized in early-stage research for antisense oligonucleotides, siRNA, and other RNA-based therapeutic platforms.
• Enzyme Kinetics: Functions as a defined sequence for measuring catalytic activity and specificity of ribonucleases and ligases.

Basic Information

Product Name	Guanylyl-(5'-3')-Cytidylyl-(5'-3')-Adenosine
CAS No.	3393-25-7
Molecular Formula	C31H38N15O19P2
Molecular Weight	986.67 g/mol
Synonyms	GpCpA; Guanosine(5')cytidine(5')adenosine triphosphate; 5'-Guanilyl-cytidyl-adenosine; GCA trinucleotide; Guanylylcytidylyladenosine; 5'-O-[Hydroxy({[hydroxy({[hydroxy(3-hydroxy-3-{[2-hydroxy-5-(6-imino-1,9-dihydro-6H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}-2,2-dimethylpropoxy)phosphoryl]oxy})phosphoryl]oxy})phosphoryl]adenosine
EINECS	Contact for details

Quality Control

Our Guanylyl-(5'-3')-Cytidylyl-(5'-3')-Adenosine is produced under controlled conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. All batches undergo rigorous analytical testing, including HPLC and mass spectrometry, to verify identity and purity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.