Description

5-Trifluoromethyl-2'-Deoxyuridine 5'-Triphosphate is a modified nucleoside triphosphate analog, a critical building block for advanced biochemical research and development. This compound is valued for its ability to incorporate a trifluoromethyl group into nucleic acid chains, offering unique properties for probing and manipulating biological systems. It is primarily utilized by researchers and developers in the fields of molecular biology, medicinal chemistry, and diagnostic assay development.

Application

• Molecular Biology Research: Used as a substrate for DNA polymerases in enzymatic synthesis, PCR, and site-directed mutagenesis studies.
• Antiviral Drug Development: Serves as a key intermediate or active metabolite in the research of nucleoside-based antiviral agents targeting viral polymerases.
• Biochemical Probes: Incorporated into oligonucleotides to create probes with enhanced stability or specific labeling for diagnostic assays and structural studies.
• Enzyme Mechanism Studies: Employed to investigate the kinetics and specificity of DNA/RNA polymerases and other nucleotide-processing enzymes.
• Medicinal Chemistry: Acts as a crucial synthon for the chemical synthesis of novel nucleoside analogs with potential therapeutic applications.
• Diagnostic Reagent Formulation: A component in the development of specialized kits for genetic testing and pathogen detection.

Basic Information

Product Name	5-Trifluoromethyl-2'-Deoxyuridine 5'-Triphosphate
CAS No.	345-03-9
Molecular Formula	C11H14F3N2O13P3
Molecular Weight	550.14 g/mol
Synonyms	5-Trifluoromethyl-dUTP; 5-CF3-dUTP; Trifluorothymidine triphosphate; F3TTP; 5-Trifluoromethyl-2'-deoxyuridine-5'-triphosphate; 1-(2-Deoxy-β-D-ribofuranosyl)-5-(trifluoromethyl)uracil 5'-triphosphate; 5-Trifluoromethyl-2'-deoxyuridine triphosphate; 5-CF3-2'-deoxyuridine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our 5-Trifluoromethyl-2'-Deoxyuridine 5'-Triphosphate is manufactured under stringent quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.