Description

Cytidine 5'-Triphosphate, Ammonium Salt (5,6-D2, 97%) 90% Chemical Purity (In Soln) is a deuterium-labeled, high-purity nucleotide essential for advanced biochemical research and diagnostic development. This compound provides a critical, isotopically enriched substrate for mechanistic studies, metabolic pathway tracing, and the synthesis of labeled nucleic acids. It is primarily utilized by research scientists and process developers in pharmaceutical R&D, biotechnology, and academic institutions focused on enzymology, virology, and NMR-based structural analysis.

Application

• Biochemical Research & Enzymology: Used as a deuterated substrate for kinetic and mechanistic studies of RNA polymerases, kinases, and other nucleotide-dependent enzymes.
• Metabolic Pathway Analysis: Employed in tracer studies using NMR or mass spectrometry to investigate nucleotide metabolism and biosynthesis in cell cultures.
• Labeled Nucleic Acid Synthesis: Serves as a building block for the enzymatic synthesis of deuterium-labeled RNA or DNA oligonucleotides for structural biology applications.
• Pharmaceutical R&D: Utilized in drug discovery programs targeting viral polymerases (e.g., HCV, SARS-CoV-2) and in the development of nucleotide-based therapeutics.
• Diagnostic Assay Development: Acts as a key component in the formulation of kits for molecular diagnostics and high-sensitivity detection methods.
• NMR Spectroscopy: Provides a valuable tool for biomolecular structure determination and dynamics studies due to the deuterium labels, which simplify complex spectra.

Basic Information

Product Name	Cytidine 5'-Triphosphate, Ammonium Salt (5,6-D2, 97%) 90% Chemical Purity (In Soln)
CAS No.	2483831-93-0
Molecular Formula	C9H13D2N3O14P3 · xNH3
Molecular Weight	Contact for details
Synonyms	CTP Ammonium Salt (5,6-D2); Deuterated Cytidine Triphosphate; 5,6-Dideutero-CTP; Cytidine-5'-triphosphate-5,6-D2 ammonium salt; [(2R,3S,4R,5R)-5-(4-Amino-5,6-dideutero-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl (hydroxy-phosphonooxyphosphoryl) hydrogen phosphate ammonium salt; Ammonium CTP-d2; CTP-d2 (Ammonium); Labeled Nucleotide Triphosphate
EINECS	Contact for details

Quality Control

This high-grade biochemical is produced under strict quality control protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry to confirm isotopic enrichment, chemical purity, and identity. A comprehensive Certificate of Analysis (COA) detailing purity (HPLC), deuterium enrichment (NMR/MS), pH, and solution concentration is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. The solution should be aliquoted to avoid repeated freeze-thaw cycles. Keep the container in a dry, cool, and well-ventilated place.

Specification

Item	Specification
Appearance (Solution)	Clear, colorless to slightly yellow
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 90% (Chemical Purity)
Deuterium Enrichment (NMR/MS)	≥ 97% (5,6-D2)
pH	6.0 - 8.0
Solvent	Water or specified buffer
Concentration	Verified by UV absorbance
Biological Activity	Functional in enzymatic assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.