Description

Sulfo-Cy5 NHS Methyl Type | Lumiprobe Type is a water-soluble, near-infrared (NIR) fluorescent labeling reagent featuring a reactive NHS ester group. This compound is engineered for the efficient, covalent labeling of primary amines (e.g., lysine residues) on proteins, peptides, antibodies, and other biomolecules, enabling sensitive detection and analysis. It is an essential tool for researchers and developers in bioconjugation, flow cytometry, fluorescence microscopy, and in vivo imaging applications, where its sulfonate groups provide enhanced solubility and reduce dye aggregation.

Application

• Antibody and Protein Labeling: Conjugation to monoclonal antibodies, enzymes, and other proteins for immunoassays (ELISA, Western blot) and diagnostic probe development.
• Flow Cytometry & Cell Analysis: Fluorescent tagging of cell surface proteins or intracellular targets for multicolor flow cytometry and cell sorting.
• Fluorescence Microscopy & Imaging: Preparation of labeled probes for fixed-cell and live-cell imaging studies due to its photostability and NIR emission.
• In Vivo Imaging: Labeling of targeting molecules (e.g., peptides, antibodies) for non-invasive optical imaging in preclinical research.
• Nucleic Acid Labeling: Modification of amine-modified oligonucleotides for fluorescence in situ hybridization (FISH) and other genomic techniques.
• Biomaterial and Nanoparticle Functionalization: Covalent attachment to amine-coated microspheres, quantum dots, or other nanomaterials to create fluorescent probes.

Basic Information

Product Name	Sulfo-Cy5 NHS Methyl Type
CAS No.	2230212-27-6
Molecular Formula	C41H47KN4O11S2
Molecular Weight	899.06 g/mol
Synonyms	Sulfo-Cyanine5 NHS ester; Sulfo-Cy5 NHS Ester Potassium Salt; Cyanine5 sulfonyl NHS ester; Sulfo-Cy5 SE; 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-2-((1E,3E)-5-((1E,3E)-3-(3-(3-sulfopropyl)-2,3-dihydro-1H-benzo[e]indol-2-yl)allylidene)-4-oxocyclopent-1-en-1-yl)vinyl)-3-(3-sulfopropyl)-1H-benzo[e]indol-1-ium potassium salt; Lumiprobe Cy5 NHS ester sulfonated; Water-soluble Cy5 NHS ester; NIR fluorescent dye NHS ester.
EINECS	Contact for details

Quality Control

Every batch of Sulfo-Cy5 NHS Methyl Type is manufactured under strict quality guidelines and undergoes comprehensive analytical testing to ensure high purity and consistent performance. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data for identity (HPLC, MS), purity (HPLC), and functional validation. Our quality system supports compliance with relevant standards for research and in vitro diagnostic (IVD) applications.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	646 - 652 nm
Emission (λmax)	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Water Content (KF)	≤ 5.0 %
Functional Activity (Labeling)	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.