Description

5'-o-(4,4'-Dimethoxytrityl)-5-Methoxyuridine CAS NO 2095417-73-3 is a key protected nucleoside derivative, specifically a 5-methoxyuridine building block with a dimethoxytrityl (DMT) group at the 5'-hydroxyl position. This protection is critical for controlling regioselectivity during automated oligonucleotide synthesis, ensuring high coupling efficiency and purity. It is an essential intermediate for research and production in the fields of antisense oligonucleotides, siRNA, and therapeutic nucleic acid development, where precise chemical modification is paramount.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): As a phosphoramidite precursor for the controlled incorporation of modified nucleosides into DNA/RNA strands.
• Antisense Oligonucleotide (ASO) Manufacturing: A critical building block for creating therapeutic ASOs targeting specific mRNA sequences.
• Small Interfering RNA (siRNA) Production: Used in the synthesis of chemically modified siRNA duplexes to enhance stability and efficacy.
• Research & Development of Nucleic Acid Therapeutics: Enables the exploration of novel nucleoside analogs for improved pharmacokinetic properties.
• Diagnostic Probe Synthesis: For creating labeled or modified nucleic acid probes used in molecular diagnostics and hybridization assays.
• Pharmaceutical Intermediate: Serves as a high-purity starting material for the synthesis of more complex nucleoside-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	5'-o-(4,4'-Dimethoxytrityl)-5-Methoxyuridine
CAS No.	2095417-73-3
Molecular Formula	C32H34N2O8
Molecular Weight	574.62 g/mol
Synonyms	5'-O-DMT-5-Methoxyuridine; 5-Methoxyuridine, 5'-O-(4,4'-dimethoxytrityl)-; 5'-O-(4,4'-Dimethoxytrityl)-5-methoxyuridine; DMT-5-Methoxyuridine; 5-Methoxy-2'-deoxyuridine 5'-(4,4'-dimethoxytrityl) ether; 5-Methoxyuridine DMT ether; 5'-DMT-5-Me-uridine; Protected 5-Methoxyuridine
EINECS	Contact for details

Quality Control

Our production of 5'-o-(4,4'-Dimethoxytrityl)-5-Methoxyuridine adheres to stringent quality protocols suitable for pharmaceutical intermediate applications. Each batch is characterized and tested to ensure identity, purity, and consistency. Certificates of Analysis (COA) are provided, detailing specifications such as assay (HPLC), related substances, and moisture content. We support cGMP compliance for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive); therefore, containers should be sealed under an inert atmosphere (e.g., nitrogen or argon) after opening and stored in a desiccator to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Assay (HPLC)	≥ 98.0%
Purity (HPLC, Area%)	≥ 99.0%
Related Substances (HPLC)	Individual impurity ≤ 0.5%
Total Impurities	≤ 1.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.