Description

Fluorescein-dUTP *1 mM in Tris Buffer (pH 7.5)* is a ready-to-use, fluorescently labeled nucleotide solution designed for enzymatic incorporation into nucleic acids. This product is essential for high-sensitivity detection and quantification in molecular biology assays, offering superior brightness and photostability. It is a critical reagent for researchers and diagnostic developers in life sciences, genomics, and clinical diagnostics requiring robust, non-radioactive labeling.

Application

• Fluorescent In Situ Hybridization (FISH): Direct labeling of DNA probes for chromosome analysis and pathogen detection.
• DNA Microarray Fabrication: Synthesis of fluorescently tagged cDNA or cRNA targets for gene expression profiling.
• Next-Generation Sequencing (NGS) Library Preparation: Incorporation into adapters or amplicons for library quantification and quality control.
• Polymerase Chain Reaction (PCR) and qPCR: Generation of fluorescently labeled PCR products for fragment analysis or as internal standards.
• Nick Translation and Random Priming: Efficient labeling of DNA for use as high-specific-activity probes.
• Apoptosis Detection (TUNEL Assay): Enzymatic labeling of DNA strand breaks in fixed cells or tissues.
• Single Nucleotide Polymorphism (SNP) Genotyping: Used in primer extension assays for allele discrimination.

Basic Information

Product Name	Fluorescein-dUTP *1 mM in Tris Buffer (pH 7.5)*
CAS No.	214154-36-6
Molecular Formula	C36H38N3O22P3
Molecular Weight	977.61 g/mol
Synonyms	Fluorescein-12-dUTP; Fluorescein-12-2'-deoxyuridine-5'-triphosphate; 5-[(2'-Deoxyuridin-5'-)triphosphoryl]-fluorescein-12-UTP; Fluorescein-12-2'-deoxyuridine-5'-triphosphate, trisodium salt; FITC-dUTP; Fluorescein-labeled dUTP; dUTP-fluorescein
EINECS	Contact for details

Quality Control

Our Fluorescein-dUTP solution is manufactured under stringent quality systems. Each batch is tested to ensure high performance and lot-to-lot consistency for critical applications. Comprehensive Certificates of Analysis (COA) detailing purity, concentration, and functional performance are provided to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The solution is supplied in Tris buffer at pH 7.5 for optimal enzymatic compatibility.

Specification

Item	Specification
Appearance	Clear orange solution
Concentration	1 mM dUTP (nominal)
Buffer	10 mM Tris-HCl, pH 7.5
Excitation Maximum	~495 nm
Emission Maximum	~520 nm
Purity (HPLC)	≥ 95%
Functional Test	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.