Description

Dmt-Loct Amidite 0.25G, Abi, Single CAS NO 206055-75-6 is a high-purity, single-use amidite reagent specifically designed for automated oligonucleotide synthesis. This product is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA and RNA oligonucleotides. It is an essential raw material for research institutions, diagnostic developers, and pharmaceutical companies engaged in molecular biology, genomics, and therapeutic nucleic acid development.

Application

• Oligonucleotide Synthesis: Primary building block for the solid-phase synthesis of DNA and RNA strands on automated synthesizers.
• Therapeutic Nucleic Acid Production: Manufacture of antisense oligonucleotides, siRNA, and aptamers for pharmaceutical applications.
• Diagnostic Probe Development: Synthesis of labeled probes for PCR, qPCR, FISH, and microarray technologies.
• Research-Grade Oligo Synthesis: Production of primers, linkers, and gene fragments for academic and industrial research.
• Next-Generation Sequencing (NGS): Fabrication of custom oligo pools and adapters used in sequencing library preparation.
• CRISPR Guide RNA Synthesis: Chemical synthesis of single-guide RNA (sgRNA) components for gene editing workflows.

Basic Information

Product Name	Dmt-Loct Amidite 0.25G, Abi, Single CAS NO 206055-75-6
CAS No.	206055-75-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dT-CE Phosphoramidite; Thymidine β-cyanoethyl phosphoramidite; DMT-dT-CE Phosphoramidite; Amidite T; dT Phosphoramidite; ABz dT Amidite; Deoxythymidine Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of Dmt-Loct Amidite is manufactured and tested under strict quality management systems to ensure it meets the exacting standards required for oligonucleotide synthesis. Our products undergo rigorous quality testing, including HPLC purity analysis and 31P NMR spectroscopy, to ensure compliance with industry standards and guarantee optimal coupling efficiency. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store the sealed vial in a freezer at -20°C under anhydrous conditions. The product is hygroscopic (moisture-sensitive) and must be handled in a dry atmosphere or with proper desiccation. Allow the vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.