Description

5'(6)-Fam CAS NO 204697-37-0 is a high-purity fluorescent labeling reagent, specifically a carboxyfluorescein derivative. This compound is essential for creating highly sensitive and stable fluorescent probes and conjugates used in advanced molecular detection and analysis. It is a critical material for researchers and manufacturers in the life sciences, diagnostic assay development, and oligonucleotide synthesis sectors, enabling precise tagging for techniques like fluorescence in situ hybridization (FISH) and real-time PCR.

Application

• Oligonucleotide Labeling: Primary reagent for the 5' or 3' end-labeling of DNA/RNA probes with a bright fluorescein derivative.
• Diagnostic Assay Development: Used in the synthesis of fluorescent conjugates for immunoassays (ELISA, lateral flow) and clinical diagnostics.
• Fluorescence In Situ Hybridization (FISH): Key component for preparing high-sensitivity FISH probes for cytogenetics and pathology.
• Real-time PCR Probes: Serves as a fluorescent reporter dye in the construction of TaqMan®-style or molecular beacon probes.
• Bioconjugation: Active ester for labeling antibodies, proteins, peptides, and other biomolecules with a stable fluorescent tag.
• Cell Biology & Imaging: Used to prepare fluorescent tracers for cellular uptake studies and microscopic imaging.
• Next-Generation Sequencing (NGS): Employed in certain sequencing-by-synthesis platforms as a cleavable fluorescent terminator.

Basic Information

Product Name	5'(6)-Fam CAS NO 204697-37-0
CAS No.	204697-37-0
Molecular Formula	C24H19NO7
Molecular Weight	433.41 g/mol
Synonyms	5(6)-Carboxyfluorescein N-succinimidyl ester; 6-FAM, SE; 5-FAM, SE; FAM NHS ester; 5(6)-Carboxyfluorescein succinimidyl ester; Fluorescein-5(6)-isothiocyanate isomer; 5(6)-FAM, SE; 6-Carboxyfluorescein N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Our 5'(6)-Fam is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. We provide a detailed Certificate of Analysis (COA) with every shipment, confirming it meets the specifications required for sensitive life science applications.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95.0% (Anhydrous basis)
Water Content (KF)	≤ 2.0%
Solubility	Clear, yellow to orange solution in DMF or DMSO
Functional Performance	Conjugation efficiency ≥ 90% (tested with standard amine)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.