Description

Guanosine 5'-(Tetrahydrogen Triphosphate), 2'-Deoxy-2'-Fluoro- is a chemically modified nucleotide triphosphate analog where the 2'-hydroxyl group of the ribose sugar is replaced by a fluorine atom. This modification confers significant resistance to enzymatic degradation, making it a critical building block for the synthesis of stable RNA and oligonucleotides. It is primarily utilized by research institutions and biotechnology companies engaged in therapeutic oligonucleotide development, structural biology studies, and advanced molecular diagnostics.

Application

• Therapeutic Oligonucleotide Synthesis: Key monomer for producing nuclease-resistant antisense oligonucleotides (ASOs), siRNA, and aptamers with enhanced stability and pharmacokinetic profiles.
• Structural Biology & Biochemistry: Used in X-ray crystallography and NMR studies to investigate RNA-protein interactions and enzyme mechanisms by trapping catalytic intermediates.
• Diagnostic Probe Development: Incorporation into fluorescent or labeled probes for in situ hybridization (FISH) and PCR-based assays, improving probe longevity and signal specificity.
• Enzymology Research: Substrate for studying the specificity and kinetics of polymerases, kinases, and other nucleic acid-metabolizing enzymes.
• SELEX Technology: Essential for generating modified RNA libraries to select for high-affinity, nuclease-resistant aptamers for therapeutic or sensing applications.
• RNA Labeling: Serves as a precursor for the enzymatic incorporation of reporter groups (e.g., biotin, fluorophores) into RNA molecules.

Basic Information

Product Name	Guanosine 5'-(Tetrahydrogen Triphosphate), 2'-Deoxy-2'-Fluoro-
CAS No.	202186-97-8
Molecular Formula	C10H16FN5O13P3
Molecular Weight	559.16 g/mol
Synonyms	2'-Fluoro-2'-deoxyguanosine-5'-triphosphate; 2'-F-dGTP; 2'-Fluoro-2'-deoxy-GTP; 2'-F-GTP; 2'-Deoxy-2'-fluoroguanosine 5'-triphosphate; Guanosine, 2'-deoxy-2'-fluoro-, 5'-(tetrahydrogen triphosphate); 2'-Fluoro-2'-deoxyguanosine triphosphate; 2'-F-2'-dGTP
EINECS	Contact for details

Quality Control

Our high-purity nucleotide analogs are manufactured under strict quality systems. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and biological activity. We provide comprehensive Certificates of Analysis (COA) detailing purity, impurity profiles, and solvent content. Production can adhere to GMP-grade standards for therapeutic development upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Anion Exchange or Reverse Phase)
Assay (Enzymatic)	≥ 90% (as triphosphate)
Water Content (KF)	≤ 5%
pH (1% Solution)	6.0 - 7.5
Endotoxin	< 10 EU/mg (for applicable grades)
Biological Activity	Suitable for polymerase incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.