Description

2',4'-Dinitrophenylglucopyranoside is a specialized glycoside derivative where a dinitrophenyl group is linked to a glucopyranose sugar moiety. This compound is primarily valued as a chromogenic substrate for the detection and assay of α-glucosidase and related hydrolytic enzyme activity. It is essential for research and quality control laboratories in the life sciences, pharmaceutical development, and diagnostic manufacturing sectors, where precise enzymatic analysis is critical.

Application

• Enzymatic Substrate: Acts as a chromogenic substrate for the spectrophotometric assay of α-glucosidase activity in biochemical research.
• Diagnostic Reagent: Used in the formulation of diagnostic kits for the detection of enzymatic disorders, such as Pompe disease.
• Pharmaceutical Research: Employed in high-throughput screening (HTS) to identify and characterize potential inhibitors of carbohydrate-processing enzymes for drug discovery.
• Microbiology: Utilized in microbial identification systems that rely on specific glycosidase activity patterns.
• Food & Beverage Analysis: Applied in quality control to measure enzyme activity in products like brewing and baking ingredients.
• Biochemical Tool: Serves as a model substrate in enzymology studies to investigate reaction kinetics and mechanisms of glycoside hydrolases.

Basic Information

Product Name	2',4'-Dinitrophenylglucopyranoside
CAS No.	25775-97-7
Molecular Formula	C12H14N2O9
Molecular Weight	330.25 g/mol
Synonyms	2,4-Dinitrophenyl α-D-glucopyranoside; α-D-Glucopyranoside, 2,4-dinitrophenyl; DNP-α-Glucoside; 2,4-DNP-α-Glc; 2,4-Dinitrophenyl-α-D-glucoside; p-Nitrophenyl α-D-glucopyranoside analog; Chromogenic α-glucosidase substrate
EINECS	Contact for details

Quality Control

Our 2',4'-Dinitrophenylglucopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Yellow to light brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.