Description

2',4'-Dinitrophenyl-β-Galactopyranoside is a specialized chromogenic and fluorogenic substrate used extensively in enzymatic assays. This compound is critical for the detection and quantification of β-galactosidase activity, a common reporter enzyme in molecular biology and diagnostic applications. It is an essential reagent for researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors, where precise enzyme measurement is paramount.

Application

• β-Galactosidase Reporter Gene Assays: A key substrate for detecting and quantifying β-galactosidase expression in molecular biology research, including gene regulation studies and promoter activity analysis.
• Diagnostic Kit Manufacturing: Used as a core component in the production of diagnostic kits for enzymatic activity detection, particularly in microbiology and cell-based assays.
• Enzyme Kinetics & Inhibition Studies: Employed in biochemical research to study enzyme kinetics, mechanism of action, and to screen for potential inhibitors of β-galactosidase.
• Cell & Tissue Staining (Histochemistry): Utilized in histochemical staining procedures to visualize β-galactosidase activity in cells and tissues, often in transgenic animal models.
• High-Throughput Screening (HTS): Serves as a reliable substrate in automated HTS platforms for drug discovery and functional genomics.
• Quality Control in Biotechnology: Applied in QC laboratories to monitor and validate the activity of recombinant β-galactosidase enzymes used in various bioprocesses.

Basic Information

Product Name	2',4'-Dinitrophenyl-β-Galactopyranoside
CAS No.	25775-96-6
Molecular Formula	C12H14N2O10
Molecular Weight	346.25 g/mol
Synonyms	DNP-β-Gal; 2,4-Dinitrophenyl β-D-galactopyranoside; o-Nitrophenyl-β-D-galactopyranoside (related); β-Galactosidase Substrate; Chromogenic Galactoside Substrate; DNP-Gal; 4-Nitrophenyl β-D-galactopyranoside (analog); ONPG (analog)
EINECS	Contact for details

Quality Control

Our 2',4'-Dinitrophenyl-β-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. We provide comprehensive Certificates of Analysis (COA) with each batch, detailing key parameters such as purity, identity, and suitability for enzymatic applications. Our quality commitment aligns with the standards expected for research and diagnostic-grade biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Yellow to light brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Suitability for Enzyme Assay	Pass

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.