Description

Poly T CAS NO 25086-81-1 is a high-purity synthetic oligonucleotide, specifically a homopolymer of thymidine nucleotides. This product is essential as a critical reagent and standard in molecular biology research and diagnostic assay development. It is primarily needed by biotechnology companies, academic research institutions, and pharmaceutical R&D departments working on nucleic acid hybridization, primer design, and quality control processes.

Application

• Molecular Biology Standards: Used as a calibration standard and positive control in polymerase chain reaction (PCR), quantitative PCR (qPCR), and sequencing applications.
• Hybridization Studies: Serves as a model homopolymer for investigating nucleic acid binding kinetics, duplex stability, and specificity in probe design.
• Primer and Probe Synthesis: Acts as a foundational component or spacer in the custom synthesis of primers, probes, and other oligonucleotides for diagnostic kits.
• Biochemical Research: Employed in studies of enzyme kinetics for polymerases, nucleases, and other nucleic acid-modifying enzymes.
• Diagnostic Development: Utilized in the development and validation of molecular diagnostic assays for genetic testing and pathogen detection.
• Quality Control: Functions as a reference material to assess the performance and purity of other oligonucleotide synthesis batches.

Basic Information

Product Name	Poly T
CAS No.	25086-81-1
Molecular Formula	C10H13N2O7P (monomer)
Molecular Weight	304.20 g/mol (monomer) *
Synonyms	Poly(thymidylic acid)
Polydeoxythymidylic acid
Polythymidylic acid
dT Homopolymer
Oligo(dT)
Poly(dT)
Thymidine Homopolymer
EINECS	Contact for details

* Molecular weight varies with polymer chain length (n). Please contact us for the specific molecular weight of your desired grade.

Quality Control

Our Poly T is manufactured under stringent conditions to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, mass spectrometry for identity confirmation, and spectrophotometry for concentration verification. Certificates of Analysis (COA) detailing purity, endotoxin levels, and functional performance data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to prevent condensation. For in-use solutions, prepare with nuclease-free water or buffer and store aliquots at -20°C to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay (UV)	90 - 110% of stated amount
Chain Length (n)	12 - 18, 18 - 24, 24 - 30, or Custom
Endotoxin Level	< 10 EU/mg
Solubility	Soluble in nuclease-free water or TE buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.