Description

o-Nitrophenyl 2-Acetamido-2-Deoxy-α-D-Galactopyranoside is a specialized chromogenic substrate used in enzymatic assays. This compound matters for its high specificity in detecting and quantifying the activity of α-N-acetylgalactosaminidase and related glycosidase enzymes. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in glycobiology research, enzyme characterization, and the development of diagnostic tests for genetic disorders like Schindler disease.

Application

• Enzymatic Research & Assay Development: As a chromogenic substrate for α-N-acetylgalactosaminidase (α-NAGA) in kinetic studies and inhibitor screening.
• Diagnostic Manufacturing: A key component in the formulation of diagnostic kits for the detection of lysosomal storage disorders, such as Schindler/Kanzaki disease.
• Glycobiology Studies: Used to investigate glycosidase specificity, carbohydrate metabolism, and enzyme mechanisms in complex biological systems.
• Biochemical Reagent: Serves as a high-purity reference standard and critical raw material in life science research and quality control laboratories.
• Pharmaceutical R&D: Employed in drug discovery programs targeting glycosidase enzymes for therapeutic intervention.

Basic Information

Product Name	o-Nitrophenyl 2-Acetamido-2-Deoxy-α-D-Galactopyranoside
CAS No.	23646-67-5
Molecular Formula	C14H18N2O8
Molecular Weight	342.30 g/mol
Synonyms	4-Nitrophenyl 2-acetamido-2-deoxy-α-D-galactopyranoside; PNP-α-GalNAc; α-GalNAc-pNP; o-Nitrophenyl N-acetyl-α-D-galactosaminide; 2-Acetamido-2-deoxy-α-D-galactopyranosyl o-nitrophenyl ether; N-Acetyl-α-D-galactosaminide 4-nitrophenyl; α-N-Acetylgalactosaminide 4-nitrophenyl
EINECS	Contact for details

Quality Control

Our o-Nitrophenyl 2-Acetamido-2-Deoxy-α-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive biochemical applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines to support research and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.