Description

Propargyl-Peg1-Acetic Acid-Nhs Ester is a heterobifunctional crosslinking reagent featuring a propargyl group for copper-catalyzed azide-alkyne cycloaddition (CuAAC) and an N-hydroxysuccinimide (NHS) ester for efficient amine coupling. This compound is essential for creating stable, site-specific bioconjugates, particularly in the development of antibody-drug conjugates (ADCs) and protein-polymer hybrids. It is a critical tool for researchers and manufacturers in the fields of biopharmaceuticals, diagnostics, and advanced materials science who require precise and reliable conjugation chemistry. The product is supplied as a high-purity reagent to ensure optimal reaction performance and reproducibility.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via the propargyl handle, enabling the creation of next-generation, homogeneous ADCs with improved therapeutic indices.
• Protein and Peptide Labeling: Functionalization of amine-containing biomolecules (e.g., proteins, peptides) with fluorescent dyes, biotin, or other probes for imaging, detection, and pull-down assays.
• Polymer Bioconjugation: Grafting of functional polymers (e.g., PEGylation) onto proteins or surfaces to enhance stability, solubility, and pharmacokinetic properties.
• Surface Modification and Immobilization: Creating functionalized surfaces for biosensors, microarrays, and affinity chromatography by immobilizing biomolecules through amine-reactive chemistry.
• Click Chemistry Scaffold: Serving as a versatile building block in combinatorial chemistry and library synthesis for drug discovery, utilizing the robust alkyne-azide "click" reaction.
• Diagnostic Reagent Development: Conjugation of enzymes or other signal-generating molecules to antibodies or antigens for use in ELISA and other immunoassay formats.

Basic Information

Product Name	Propargyl-Peg1-Acetic Acid-Nhs Ester
CAS No.	1858242-47-3
Molecular Formula	C₁₂H₁₅NO₆
Molecular Weight	269.25 g/mol
Synonyms	Propargyl-PEG1-NHS ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-(prop-2-yn-1-yloxy)ethoxy]ethoxy]propanoate; NHS-PEG1-Propargyl; Propargyl-PEG1-acid NHS ester; N-(3-[2-[2-(Prop-2-yn-1-yloxy)ethoxy]ethoxy]propionyloxy)succinimide; Propargyl-O-(CH₂CH₂O)₂-CH₂CH₂-COOH NHS ester; Alkyne-PEG1-NHS ester
EINECS	Contact for details

Quality Control

Our Propargyl-Peg1-Acetic Acid-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive bioconjugation applications. Standard quality checks include HPLC for purity assessment, NMR for structural confirmation, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.