Description

Fluorescein-PEG6-NHS Ester is a high-purity, amine-reactive fluorescent labeling reagent designed for bioconjugation. This compound is critical for researchers and manufacturers who require stable, water-soluble fluorescent probes for tracking biomolecules in complex biological systems. It is essential for applications in pharmaceutical development, diagnostic assay manufacturing, and advanced life science research where precise visualization and quantification are paramount.

Application

• Antibody-Drug Conjugate (ADC) Development: Fluorescent labeling of antibodies and linker-payloads for pharmacokinetic and biodistribution studies.
• Diagnostic Assay Manufacturing: Conjugation to proteins, peptides, or oligonucleotides to create fluorescent detection probes for ELISA, lateral flow assays, and in-situ hybridization.
• Cell Imaging and Tracking: Labeling of cell surface proteins or internalized nanoparticles for real-time visualization using fluorescence microscopy and flow cytometry.
• Protein-Protein Interaction Studies: Site-specific labeling of purified proteins for use in Fluorescence Resonance Energy Transfer (FRET) and other proximity-based assays.
• Polymer and Material Science: Functionalization of polymers, hydrogels, or nanomaterials to create traceable and smart materials for biomedical applications.
• High-Throughput Screening (HTS): Development of fluorescently tagged targets or reagents for automated drug discovery platforms.

Basic Information

Product Name	Fluorescein-PEG6-NHS Ester
CAS No.	1818294-35-7
Molecular Formula	C44H51N3O17
Molecular Weight	917.9 g/mol
Synonyms	5(6)-FAM-PEG6-NHS Ester; 5/6-Carboxyfluorescein-PEG6-NHS Ester; Fluorescein-5(6)-PEG6-NHS; 6-(Fluorescein-5(6)-carboxamido)hexanoic acid, 2,5-dioxo-1-pyrrolidinyl ester, PEGylated; 5(6)-FAM-PEG6-OSu; Succinimidyl ester of 5(6)-carboxyfluorescein-PEG6; NHS-Fluorescein-PEG6; Fluorescein PEG NHS Ester (6-unit PEG spacer)
EINECS	Contact for details

Quality Control

Every batch of Fluorescein-PEG6-NHS Ester is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high purity and consistent performance through HPLC, NMR, and mass spectrometry analysis. Certificates of Analysis (COA) detailing purity, identity, and functional performance are provided with each shipment to support your regulatory and R&D documentation requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term storage, keep containers sealed under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Single Isomer Content (HPLC)	≥ 90% (5- or 6-isomer)
Water Content (KF)	≤ 1.0%
Functional Performance	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.