Description

Chloroacetamido-Peg4-Nhs Ester CAS NO 1353011-95-6 is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a chloroacetamide group for thioether conjugation and an NHS ester for efficient amine coupling. This compound is engineered to enable the stable, site-specific bioconjugation of biomolecules, which is critical for developing advanced therapeutics and diagnostics. It is an essential reagent for researchers and manufacturers in the fields of biopharmaceuticals, antibody-drug conjugate (ADC) development, and diagnostic assay formulation.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via cysteine residues, creating next-generation ADCs with improved stability and therapeutic index.
• Protein and Peptide PEGylation: To modify therapeutic proteins and peptides with a PEG chain, enhancing their solubility, stability, and pharmacokinetic profile while reducing immunogenicity.
• Biomolecule Immobilization: For the covalent attachment of proteins, antibodies, or peptides onto solid surfaces (e.g., biosensors, microarray chips, affinity resins) for diagnostic and research applications.
• Fluorescent Probe and Dye Conjugation: To label biomolecules with fluorophores or other reporter molecules for imaging, flow cytometry, and in vitro diagnostic (IVD) assays.
• Nanoparticle Functionalization: To attach targeting ligands (e.g., peptides, antibodies) to the surface of liposomes, polymeric nanoparticles, or quantum dots for targeted drug delivery and imaging.
• Crosslinking Agent for Hydrogel Formation: As a crosslinker in the synthesis of PEG-based hydrogels for 3D cell culture, tissue engineering, and controlled drug release systems.

Basic Information

Item	Detail
Product Name	Chloroacetamido-Peg4-Nhs Ester
CAS No.	1353011-95-6
Molecular Formula	C21H35ClN2O10
Molecular Weight	511.0 g/mol (approx.)
Synonyms	Chloroacetamido-PEG4-NHS ester; NHS-PEG4-Chloroacetamide; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-(2-chloroacetamido)ethoxy]ethoxy]ethoxy]ethoxy]propanoate; N-(13-Oxo-3,6,9,12-tetraoxa-2-azatetradec-1-yl)-2-chloroacetamide; Chloroacetyl-PEG4-NHS ester; Heterobifunctional PEG crosslinker, NHS-PEG4-CAA; PEG4 spacer, chloroacetamide NHS ester
EINECS	Contact for details

Quality Control

Our Chloroacetamido-Peg4-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) detailing purity, moisture content, and functional group integrity are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.