Description

N6-Benzoyl-3'-o-(4,4'-Dimethoxytrityl)-2'-o-T-Butyldimethylsilyladenosine CAS NO 1214886-17-5 is a highly protected adenosine derivative, a critical phosphoramidite precursor used in the automated synthesis of oligonucleotides. This compound is essential for ensuring high coupling efficiency and sequence fidelity during solid-phase synthesis, directly impacting the yield and purity of the final nucleic acid product. It is a vital raw material for research institutions, diagnostic kit manufacturers, and pharmaceutical companies developing therapeutic oligonucleotides, antisense drugs, and primers for molecular biology.

Application

• Oligonucleotide Synthesis: Serves as a protected adenosine (A) building block (phosphoramidite precursor) in automated solid-phase DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Manufacturing: Key intermediate in the production of antisense oligonucleotides (ASOs), siRNA, and aptamers for pharmaceutical applications.
• Diagnostic Probe Production: Used to manufacture labeled and unlabeled DNA probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Research-Grade Primer Synthesis: Enables the custom synthesis of high-purity primers for genetic sequencing, cloning, and other molecular biology techniques.
• Synthesis of Modified Nucleosides: Acts as a starting material or intermediate for further chemical modification to create novel nucleoside analogs.

Basic Information

Product Name	N6-Benzoyl-3'-o-(4,4'-Dimethoxytrityl)-2'-o-T-Butyldimethylsilyladenosine
CAS No.	1214886-17-5
Molecular Formula	C44H49N5O7Si
Molecular Weight	788.00 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-O-(tert-butyldimethylsilyl)adenosine; 2'-O-TBDMS-N6-Bz-5'-O-DMT-Adenosine; DMT-rA(Bz)-TBDMS-3'-OH; N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)adenosine; Protected Adenosine Derivative; Adenosine, N6-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-; rA(Bz) DMT TBDMS
EINECS	Contact for details

Quality Control

This high-purity nucleotide building block is manufactured under controlled environments to ensure batch-to-batch consistency suitable for sensitive oligonucleotide synthesis. Quality is verified through rigorous analytical methods including HPLC, NMR, and mass spectrometry to confirm identity, purity, and the absence of critical impurities like free nucleobases or protecting group fragments. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each batch to support your quality management and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (e.g., argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, dichloromethane, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.