Description

3′-o-Azidomethyl-n-Bz Dc is a specialized nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. This compound matters for its role in introducing specific modifications into DNA or RNA sequences, enabling advanced research and therapeutic development. It is primarily needed by biotechnology and pharmaceutical companies engaged in antisense therapy, siRNA development, and diagnostic probe manufacturing.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of modified DNA/RNA strands.
• Antisense & siRNA Therapeutics: For constructing oligonucleotides with enhanced stability and targeting capabilities for gene silencing applications.
• Diagnostic Probe Development: Used to create labeled probes for genetic testing, fluorescence in situ hybridization (FISH), and microarray technologies.
• Basic Biochemical Research: A vital reagent for studying nucleic acid structure, function, and protein-nucleic acid interactions.
• Click Chemistry Conjugation: The azido group allows for efficient, site-specific bioconjugation via copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• Synthesis of Aptamers: For creating chemically modified aptamers with improved binding affinity and nuclease resistance.

Basic Information

Product Name	3′-o-Azidomethyl-n-Bz Dc
CAS No.	1187621-03-9
Molecular Formula	C41H47N8O7P
Molecular Weight	794.85 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxycytidine, 3'-O-[(2-azidomethyl)benzoyl]-, 2-Cyanoethyl N,N-diisopropylphosphoramidite; 3'-O-(Azidomethylbenzoyl)-5'-O-DMT-2'-deoxycytidine-2-cyanoethyl N,N-diisopropylphosphoramidite; Dc(Az) Phosphoramidite; 3'-O-Azidomethylbenzoyl-5'-O-DMT-deoxycytidine CE Phosphoramidite; 2'-Deoxycytidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-O-[[(2-azidomethyl)benzoyl]-, 2-cyanoethyl N,N-diisopropylphosphoramidite
EINECS	Contact for details

Quality Control

Our 3′-o-Azidomethyl-n-Bz Dc is manufactured under strict quality systems to ensure consistency and reliability for sensitive oligonucleotide synthesis. Each batch is characterized by HPLC, NMR, and mass spectrometry to confirm identity and high purity. Certificates of Analysis (COA) with full analytical data are provided to support your GMP or research-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C ± 5°C under an inert atmosphere (argon or nitrogen). The product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.