Description

4'-Aminomethyl-6-Fam CAS NO 198546-45-1 is a specialized fluorescent dye derivative, specifically a functionalized analog of the widely used 6-carboxyfluorescein (6-FAM). This compound matters for its reactive primary amine group, which enables covalent conjugation to biomolecules, making it an essential tool for labeling and detection in advanced analytical workflows. Researchers and manufacturers in the fields of life sciences, molecular diagnostics, and pharmaceutical development require this high-purity intermediate for creating sensitive probes, assay reagents, and diagnostic kits.

Application

• Fluorescent Labeling Reagent: Primary use as an amine-reactive dye for labeling peptides, proteins, antibodies, and oligonucleotides.
• Bioconjugation Chemistry: Key intermediate in the synthesis of fluorescent probes and conjugates for bioimaging and flow cytometry.
• Diagnostic Assay Development: Used in the manufacturing of reagents for ELISA, fluorescence in situ hybridization (FISH), and other clinical diagnostic tests.
• Proteomics & Genomics Research: Enables fluorescence-based detection in gel electrophoresis, microarrays, and next-generation sequencing applications.
• High-Throughput Screening (HTS): Serves as a core component in developing fluorescent sensors and indicators for drug discovery platforms.
• Chemical Biology Tools: Building block for creating activity-based probes to study enzyme function and cellular processes.

Basic Information

Product Name	4'-Aminomethyl-6-Fam
CAS No.	198546-45-1
Molecular Formula	C26H21NO7
Molecular Weight	459.45 g/mol
Synonyms	6-FAM-AM; 6-Carboxyfluorescein Aminomethyl Derivative; 5(6)-Carboxyfluorescein-4'-aminomethyl; 4'-(Aminomethyl)fluorescein-6-carboxylic Acid; Aminomethyl-6-carboxyfluorescein; FAM-amine; Fluorescein-6-aminomethylcarboxylic Acid; 6-FAM Amine
EINECS	Contact for details

Quality Control

Our 4'-Aminomethyl-6-Fam is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, consider desiccation to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Individual impurity ≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.