Description

Biotinamidohexanoic Acid 3-Sulfo-n-Hydroxysuccinimide Ester Sodium Salt is a highly reactive, water-soluble biotinylation reagent designed for efficient labeling of biomolecules. Its core value lies in the unique combination of a long-chain biotin derivative with a sulfo-NHS ester group, enabling stable amide bond formation under mild, aqueous conditions. This compound is essential for researchers and manufacturers in the life sciences, particularly for applications in diagnostic assay development, affinity chromatography, and proteomics research where robust and specific biotin-streptavidin interactions are required.

Application

• Antibody and Protein Labeling: For conjugation to primary antibodies, enzymes, and other proteins for use in ELISA, Western blotting, and immunohistochemistry.
• Nucleic Acid Probe Conjugation: For biotinylating oligonucleotides and DNA/RNA probes for hybridization assays and microarray technologies.
• Affinity Purification Matrix Preparation: For immobilizing biotinylated ligands onto streptavidin or avidin resins for pull-down assays and protein complex isolation.
• Cell Surface Labeling: For tagging cell surface proteins and receptors for flow cytometry (FACS) and imaging studies.
• Diagnostic Kit Manufacturing: As a key reagent in the production of lateral flow assays, chemiluminescent immunoassays, and other point-of-care diagnostic devices.
• Drug Delivery System Development: For creating biotinylated nanoparticles or liposomes targeted via the streptavidin-biotin system.

Basic Information

Product Name	Biotinamidohexanoic Acid 3-Sulfo-n-Hydroxysuccinimide Ester Sodium Salt
CAS No.	191671-46-2
Molecular Formula	C20H29N4NaO9S2
Molecular Weight	556.58 g/mol
Synonyms	Sulfo-NHS-LC-Biotin; Biotinamidocaproic Acid 3-Sulfo-N-hydroxysuccinimide Ester Sodium Salt; EZ-Link™ Sulfo-NHS-LC-Biotin; Nε-(+)-Biotinyl-6-aminocaproic acid N-hydroxysulfosuccinimide ester sodium salt; Biotin-XX, SSE; 6-((6-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)hexanoyl)amino)hexanoic acid 2,5-dioxopyrrolidin-1-yl ester sulfonate sodium salt; Sulfo-NHS-Biotin (Long Chain); Biotinamidohexanoic acid sulfo-NHS ester sodium salt
EINECS	Contact for details

Quality Control

Our Biotinamidohexanoic Acid 3-Sulfo-n-Hydroxysuccinimide Ester Sodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store the product dry.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility (1% in Water)	Clear, colorless solution
Functional Assay	≥ 90.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.