Description

n-Succinimidyl 7-Hydroxy-4-Coumarinyl-Acetate is a specialized, high-purity chemical reagent designed for advanced bioconjugation and labeling applications. This compound serves as a critical building block for creating fluorescent probes and tagged biomolecules due to its reactive succinimidyl ester group and inherent coumarin fluorophore. It is essential for researchers and manufacturers in pharmaceutical development, diagnostic assay production, and advanced life science research who require reliable, high-quality labeling agents.

Application

• Fluorescent Labeling of Biomolecules: Primary application for tagging proteins, peptides, and antibodies with a blue-fluorescent coumarin dye for detection and imaging.
• Proteomics and Bioanalytical Research: Used in activity-based protein profiling (ABPP) and other chemical proteomics strategies to study enzyme function.
• Diagnostic Reagent Synthesis: Key intermediate in the manufacture of fluorescent conjugates for ELISA, lateral flow assays, and other in vitro diagnostic (IVD) kits.
• Cell Biology and Imaging: Enables the creation of cell-permeable fluorescent tracers and activity reporters for live-cell microscopy.
• Material Science Functionalization: Used to introduce fluorescent tags onto nanoparticles or polymer surfaces for tracking and characterization.
• High-Throughput Screening (HTS): Serves as a component in developing fluorescent substrates or probes for enzymatic assays in drug discovery.

Basic Information

Item	Details
Product Name	n-Succinimidyl 7-Hydroxy-4-Coumarinyl-Acetate
CAS No.	185102-64-1
Molecular Formula	C15H11NO7
Molecular Weight	317.25 g/mol
Synonyms	7-Hydroxycoumarin-4-acetic Acid N-Succinimidyl Ester; 4-Acetyl-7-hydroxycoumarin N-hydroxysuccinimide ester; NHS-7-Hydroxy-4-coumarinylacetate; Succinimidyl 7-Hydroxycoumarin-4-acetate; 7-Hydroxy-4-coumarinylacetic Acid N-Succinimidyl Ester; 4-Acetoxy-7-hydroxycoumarin NHS Ester; Coumarin NHS Ester (7-Hydroxy-4-substituted); 185102-64-1
EINECS	Contact for details

Quality Control

Every batch of n-Succinimidyl 7-Hydroxy-4-Coumarinyl-Acetate is manufactured and tested under strict quality management systems to ensure high purity and batch-to-batch consistency. Our quality control protocol includes comprehensive analytical techniques such as HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) documenting identity, purity, and impurity profiles is provided with each shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light yellow crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.