Description

N4-Benzoyl-5'-o-(4,4'-Dimethoxytrityl)-2'-o-Propargyl Adenosine is a highly specialized, protected nucleoside derivative designed for advanced oligonucleotide synthesis. This compound is a critical building block in the solid-phase synthesis of modified RNA and DNA, enabling the precise introduction of functional groups for research and therapeutic applications. It is essential for pharmaceutical R&D, academic research, and biotechnology companies focused on developing antisense oligonucleotides, siRNA, and other nucleic acid-based therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis: A key phosphoramidite precursor for the automated synthesis of RNA and DNA strands.
• Antisense Oligonucleotide (ASO) Development: Used in the research and production of therapeutic ASOs targeting genetic diseases.
• Small Interfering RNA (siRNA) Manufacturing: Enables the site-specific incorporation of propargyl groups for subsequent conjugation (e.g., with fluorophores, targeting ligands).
• Nucleic Acid Research Tools: Synthesis of labeled probes and primers for diagnostic assays and genomic studies.
• Click Chemistry Conjugation: The 2'-O-propargyl group facilitates copper-catalyzed azide-alkyne cycloaddition (CuAAC) for post-synthetic modification.
• Pharmaceutical Intermediate: Serves as a high-purity intermediate in the cGMP-compliant production of active pharmaceutical ingredients (APIs) for nucleic acid drugs.

Basic Information

Product Name	N4-Benzoyl-5'-o-(4,4'-Dimethoxytrityl)-2'-o-Propargyl Adenosine
CAS No.	171486-51-4
Molecular Formula	C43H39N5O7
Molecular Weight	737.80 g/mol
Synonyms	2'-O-Propargyl-N-benzoyl-5'-O-(4,4'-dimethoxytrityl)adenosine; 5'-O-DMT-2'-O-propargyl-N-benzoyladenosine; N-Bz-5'-O-DMT-2'-O-propargyladenosine; Adenosine, N-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-2-propynyl-; 2'-O-Propargyladenosine DMT-protected intermediate; Protected Adenosine Phosphoramidite Precursor
EINECS	Contact for details

Quality Control

Every batch of N4-Benzoyl-5'-o-(4,4'-Dimethoxytrityl)-2'-o-Propargyl Adenosine is manufactured and tested under strict quality management systems. We guarantee high purity and consistency, with specifications tailored for demanding nucleic acid synthesis applications. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), identity (by NMR and MS), and moisture content is provided with each shipment to ensure traceability and compliance with your research or cGMP requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This material is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.