Description

2',3'-Dideoxyadenosine-5'-o-(1-Thiotriphosphate) Sodium Salt is a chemically modified nucleotide analog where a sulfur atom replaces one of the non-bridging oxygen atoms in the triphosphate moiety. This compound is a critical reagent in molecular biology research, particularly for its role in chain-termination sequencing methods and as a substrate for studying DNA polymerases. It is essential for researchers and manufacturers in the fields of genomics, enzymology, and diagnostic assay development who require high-purity, chemically defined nucleotide analogs.

Application

• DNA Sequencing: Used as a chain-terminating substrate in Sanger sequencing and next-generation sequencing (NGS) methodologies.
• Enzymology Studies: Acts as a substrate analog to investigate the kinetics, fidelity, and mechanisms of DNA-dependent polymerases and reverse transcriptases.
• Diagnostic Assay Development: Employed in the synthesis of probes and primers for PCR-based diagnostic tests and detection kits.
• Antiviral Research: Serves as a biochemical tool to study the inhibition of viral replication by nucleoside analogs.
• Biochemical Synthesis: Used in the enzymatic synthesis of modified oligonucleotides for research and therapeutic applications.
• Metabolic Pathway Analysis: Aids in tracing nucleotide incorporation and metabolism within cellular systems.

Basic Information

Product Name	2',3'-Dideoxyadenosine-5'-o-(1-Thiotriphosphate) Sodium Salt
CAS No.	154902-26-8
Molecular Formula	C10H12N5O10P3S · xNa
Molecular Weight	Contact for details
Synonyms	2',3'-Dideoxyadenosine 5'-(α-thio)triphosphate sodium salt; ddATPαS sodium salt; 2',3'-Dideoxyadenosine-5'-O-(1-thiotriphosphate) trisodium salt; Adenosine, 2',3'-dideoxy-, 5'-(trihydrogen triphosphate), P-α-thio-, sodium salt; α-Thio-ddATP; Sp-ddATPαS; 2',3'-ddATPαS; 5'-O-[(R)-Hydroxy(phosphonooxy)phosphinothioyl]-2',3'-dideoxyadenosine sodium salt
EINECS	Contact for details

Quality Control

Our 2',3'-Dideoxyadenosine-5'-o-(1-Thiotriphosphate) Sodium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the stringent requirements for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated. Allow the product to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Assay (UV)	95 - 105%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, slightly soluble in ethanol
Biological Activity	Suitable for use as a polymerase substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.